MWNTs 通过超支化 PEI 功能化用于高度选择性分离 BSA。

Functionalization of MWNTs with hyperbranched PEI for highly selective isolation of BSA.

机构信息

Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 110819, China.

出版信息

Macromol Biosci. 2010 Aug 11;10(8):906-15. doi: 10.1002/mabi.200900444.

DOI:10.1002/mabi.200900444

PMID:20443184

Abstract

Cationic hyperbranched BPEI was immobilized on the surface of MWNTs via electrostatic interactions between the positively charged protonated amines within the polymer and the carboxyl groups on the chemically oxidized MWNT surface. The functionalized BPEI-MWNTs were characterized by FT-IR, TGA, TEM and surface charge analysis, and it was used as a bio-sorbent for the adsorption of proteins. CD spectra showed no conformational change of BSA during the adsorption/desorption process. A dynamic adsorption capacity of 167 mg · g⁻¹ for BSA was achieved. With a sample volume of 2.0 mL, an enrichment factor of 10 was obtained along with an adsorption efficiency of 100%, a recovery of 100%, a sampling frequency of 10 h⁻¹ and a RSD of 2.6% at 25 µg · mL⁻¹ BSA.

摘要

通过聚合物中带正电荷的质子化伯胺与化学氧化 MWNT 表面上的羧基之间的静电相互作用，将阳离子超支化 BPEI 固定在 MWNTs 表面上。功能化的 BPEI-MWNTs 通过 FT-IR、TGA、TEM 和表面电荷分析进行了表征，并用作吸附蛋白质的生物吸附剂。CD 光谱表明，在吸附/解吸过程中 BSA 没有构象变化。实现了对 BSA 的 167mg·g⁻¹的动态吸附容量。在 2.0mL 的样品体积下，获得了 10 的富集因子，吸附效率为 100%，回收率为 100%，采样频率为 10h⁻¹，在 25µg·mL⁻¹ BSA 时 RSD 为 2.6%。