Xing Yun, Xia Zuyong, Rao Jianghong
Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, CA 94305, USA.
IEEE Trans Nanobioscience. 2009 Mar;8(1):4-12. doi: 10.1109/TNB.2009.2017321. Epub 2009 Mar 16.
Semiconductor quantum dots (QDs) have captivated researchers in the biomedical field over the last decade. Compared to organic dyes and fluorescent proteins, QDs have unique optical properties such as tunable emission spectra, improved brightness, superior photostability, and simultaneous excitation of multiple fluorescence colors. Since the first successful reports on the biological use of QDs a decade ago, QDs and their bioconjugates have been successfully applied to various imaging applications including fixed cell labeling, live-cell imaging, in situ tissue profiling, fluorescence detection and sensing, and in vivo animal imaging. In this review, we will briefly survey the optical properties of QDs, the biofunctionalization strategies, and focus on their biosensing and in vivo imaging applications. We conclude with a discussion on the issues and perspectives on QDs as biosensing probes and in vivo imaging agents.
在过去十年中，半导体量子点（QDs）吸引了生物医学领域的研究人员。与有机染料和荧光蛋白相比，量子点具有独特的光学特性，如可调发射光谱、更高的亮度、卓越的光稳定性以及同时激发多种荧光颜色。自十年前首次成功报道量子点的生物学应用以来，量子点及其生物共轭物已成功应用于各种成像应用，包括固定细胞标记、活细胞成像、原位组织分析、荧光检测与传感以及体内动物成像。在这篇综述中，我们将简要概述量子点的光学特性、生物功能化策略，并重点关注其生物传感和体内成像应用。我们最后讨论了量子点作为生物传感探针和体内成像剂的问题与前景。
