[塞来昔布对Tca8113细胞系中前列腺素E2合成及COX-2和VEGF-C mRNA表达的影响]
[Effects of celecoxib on PGE2 synthesis and COX-2 and VEGF-C mRNA expression in Tca8113 cell lines].
作者信息
Li Wei-Zhong, Wang Xiao-Yan, Ding Yan-Qing
机构信息
Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):466-8.
OBJECTIVE
To investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on prostaglandin E(2) (PGE2) release and vascular endothelial growth factor C (VEGF-C) and COX-2 mRNA expression in Tca8113 cell lines.
METHODS
MTT assay was used to analyze the proliferation of Tca8113 cells. The PGE2 level was detected with enzyme-linked immunosorbent assay (ELISA), and the expressions of COX-2 and VEGF-C mRNA were examined with RT-PCR.
RESULTS
Celecoxib could induce inhibitory effects on the growth and PGE2 release in Tca8113 cells. The RT-PCR results showed that celecoxib significantly down-regulated the expression of VEGF-C mRNA, but produced a weak effect on COX-2 mRNA expression.
CONCLUSION
The inhibitory effect of celecoxib on Tca8113 cell growth and the expressions of VEGF-C and COX-2 may be related to the release of PGE2.
目的
探讨选择性环氧化酶-2（COX-2）抑制剂塞来昔布对Tca8113细胞系中前列腺素E2（PGE2）释放、血管内皮生长因子C（VEGF-C）及COX-2 mRNA表达的影响。
方法
采用MTT法分析Tca8113细胞的增殖情况。用酶联免疫吸附测定（ELISA）检测PGE2水平，用逆转录-聚合酶链反应（RT-PCR）检测COX-2和VEGF-C mRNA的表达。
结果
塞来昔布可抑制Tca8113细胞的生长及PGE2释放。RT-PCR结果显示，塞来昔布可显著下调VEGF-C mRNA的表达，但对COX-2 mRNA表达的影响较弱。
结论
塞来昔布对Tca8113细胞生长及VEGF-C和COX-2表达的抑制作用可能与PGE2的释放有关。
Zotero 插件