Jalilian Amir R, Rostampour Nima, Rowshanfarzad Pejman, Shafaii Kamaleddin, Kamali-Dehghan Mohsen, Akhlaghi Mehdi
Nuclear Medicine Research Group Agricultural, Medical and Industrial Research School (AMIRS), Karaj, Iran.
Acta Pharm. 2009 Mar;59(1):45-55. doi: 10.2478/v10007-009-0008-9.
[61Cu]diacetyl-bis(N4-methylthiosemicarbazone) ([61Cu] ATSM) was prepared using in house-made diacetyl-bis(N4-methylthiosemicarbazone) (ATSM) ligand and [61Cu]CuCl2 produced via the natZn(p, x)61Cu (180 muA proton irradiation, 22 MeV, 3.2 h) and purified by a ion chromatography method. [61Cu]ATSM radiochemical purity was >98 %, as shown by HPLC and RTLC methods. [61Cu]ATSM was administered into normal and tumor bearing rodents for up to 210 minutes, followed by biodistribution and co-incidence imaging studies. Significant tumor/non-tumor accumulation was observed either by animal sacrification or imaging. [61Cu]ATSM is a positron emission tomography (PET) radiotracer for tumor hypoxia imaging.
利用自制的双乙酰双（N4-甲基硫代半卡巴腙）（ATSM）配体和通过天然锌（p，x）61铜（180微安质子辐照，22兆电子伏特，3.2小时）产生的[61铜]氯化铜制备了[61铜]双乙酰双（N4-甲基硫代半卡巴腙）（[61铜]ATSM），并通过离子色谱法进行纯化。如高效液相色谱法和放射薄层色谱法所示，[61铜]ATSM的放射化学纯度>98%。将[61铜]ATSM注射到正常和荷瘤啮齿动物体内长达210分钟，随后进行生物分布和符合成像研究。通过动物处死或成像观察到明显的肿瘤/非肿瘤蓄积。[61铜]ATSM是一种用于肿瘤缺氧成像的正电子发射断层扫描（PET）放射性示踪剂。
