Saparrat Mario C N, Fermoselle Geraldine E, Stenglein Sebastián A, Aulicino Mónica B, Balatti Pedro A
Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Diag. 113 y 61, CC 327, 1900 La Plata, Argentina.
Mycopathologia. 2009 Jul;168(1):41-7. doi: 10.1007/s11046-009-9194-8. Epub 2009 Mar 22.
Pseudocercospora griseola is the causal agent of angular leaf spot of common bean (ALS). It has undergone parallel coevolution with its host and two major groups have been defined, "Andean" (P. griseola f. griseola) and "Mesoamerican" (P. griseola f. mesoamericana). The aim of this study was to analyze the nature and the level of the dark pigment synthesized by the representatives of each group. After 21 days of incubation on potato dextrose agar medium, P. griseola f. griseola isolate S3b developed colonies with diameters of 17.5 +/- 1.3 mm and concentric rings of pigmentation. Isolate T4 of P. griseola f. mesoamericana presented smaller colonies (9.9 +/- 0.3 mm) with a uniform dark-gray color. Both isolates, S3b and T4, produced the same pigment, a 1,8-dihydroxynaphthalene-melanin, although different in quantity and structural features as suggested by the IR spectrum. The P. griseola f. griseola isolate S3b had a higher growth rate and melanin content as well as smaller sensitivity to melanin synthesis inhibitors compared to the isolate T4 of P. griseola f. mesoamericana. These results suggest a possible link between melanin and growth in P. griseola.
灰斑拟尾孢菌是菜豆角斑病的病原菌。它与其寄主经历了平行协同进化，已定义了两个主要类群，即 “安第斯” 类群（灰斑拟尾孢菌原变种）和 “中美洲” 类群（灰斑拟尾孢菌中美变种）。本研究的目的是分析每个类群代表菌株合成的深色色素的性质和水平。在马铃薯葡萄糖琼脂培养基上培养21天后，灰斑拟尾孢菌原变种菌株S3b形成了直径为17.5±1.3毫米且带有色素同心环的菌落。灰斑拟尾孢菌中美变种菌株T4的菌落较小（9.9±0.3毫米），呈均匀的深灰色。尽管红外光谱表明S3b和T4这两个菌株产生的色素在数量和结构特征上有所不同，但它们产生的是同一种色素，即1,8 - 二羟基萘黑色素。与灰斑拟尾孢菌中美变种菌株T4相比，灰斑拟尾孢菌原变种菌株S3b具有更高的生长速率和黑色素含量，并且对黑色素合成抑制剂的敏感性更低。这些结果表明在灰斑拟尾孢菌中黑色素与生长之间可能存在联系。
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