Instituto de Fisiología Vegetal, CCT-La Plata-Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata, Diag 113 y 61, CC 327, 1900 La Plata, Argentina.
Mycopathologia. 2012 Dec;174(5-6):397-408. doi: 10.1007/s11046-012-9558-3. Epub 2012 Jun 20.
Cladosporium cladosporioides is a dematiaceous fungus with coloured mycelia and conidia due to the presence of dark pigments. The purpose of this study was to characterize the dark pigments synthetized by Cladosporium sp. LPSC no. 1088 and also to identify the putative polyketide synthase (pks) gene that might be involved in the pigment biosynthesis. Morphological as well as molecular features like the ITS sequence confirmed that LPSC 1088 is Cladosporium cladosporioides. UV-visible, Fourier Transform Infrared (FTIR) and Electron Spin Resonance (ESR) spectroscopy analysis as well as melanin inhibitors suggest that the main dark pigment of the isolate was 1,8 dihydroxynaphthalene (DHN)-melanin-type compound. Two commercial fungicides, Difenoconazole and Chlorothalonil, inhibited fungal growth as well as increased pigmentation of the colonies suggesting that melanin might protect the fungus against chemical stress. The pigment is most probably synthetized by means of a pentaketide pathway since the sequence of a 651 bp fragment, coding for a putative polyketide synthase, is highly homologous to pks sequences from other fungi.
枝孢属 Cladosporium cladosporioides 是一种暗色真菌，其菌丝和分生孢子具有颜色，这是由于存在深色色素。本研究的目的是对 Cladosporium sp. LPSC no. 1088 合成的深色色素进行表征，并鉴定可能参与色素生物合成的假定聚酮合酶 (pks) 基因。形态学和分子特征，如 ITS 序列，证实 LPSC 1088 是枝孢属 Cladosporium cladosporioides。紫外可见、傅里叶变换红外 (FTIR) 和电子自旋共振 (ESR) 光谱分析以及黑色素抑制剂表明，该分离物的主要深色色素是 1,8 二羟基萘 (DHN)-黑色素型化合物。两种商业杀菌剂，咯菌腈和百菌清，抑制了真菌的生长并增加了菌落的色素沉着，这表明黑色素可能保护真菌免受化学应激。由于编码假定聚酮合酶的 651 bp 片段的序列与其他真菌的 pks 序列高度同源，因此该色素很可能是通过五酮途径合成的。
