Huggett Megan J, Nedved Brian T, Hadfield Michael G
University of Hawaii, Honolulu, 96813, USA.
Biofouling. 2009;25(5):387-99. doi: 10.1080/08927010902823238.
Hydroides elegans is a major fouling organism in tropical waters around the world, including Pearl Harbor, Hawaii. To determine the importance of initial surface characteristics on biofilm community composition and subsequent colonization by larvae of H. elegans, the settlement and recruitment of larvae to biofilmed surfaces with six different initial surface wettabilities were tested in Pearl Harbor. Biofilm community composition, as determined by a combined approach of denaturing gradient gel electrophoresis and fluorescence in situ hybridization, was similar across all surfaces, regardless of initial wettability, and all surfaces had distinct temporal shifts in community structure over a 10 day period. Larvae settled and recruited in higher numbers to surfaces with medium to low wettability in both May and August, and also to slides with high wettability in August. Pearl Harbor biofilm communities developed similarly on a range of surface wettabilities, and after 10 days in Pearl Harbor all surfaces were equally attractive to larvae of Hydroides elegans, regardless of initial surface properties.
优美盘管虫是世界热带水域（包括夏威夷珍珠港）的主要污损生物。为了确定初始表面特性对生物膜群落组成以及随后优美盘管虫幼虫定殖的重要性，在珍珠港对具有六种不同初始表面润湿性的生物膜表面上幼虫的附着和招募情况进行了测试。通过变性梯度凝胶电泳和荧光原位杂交相结合的方法确定的生物膜群落组成在所有表面上都相似，无论初始润湿性如何，并且在10天的时间内所有表面的群落结构都有明显的时间变化。在五月和八月，幼虫在中低润湿性的表面上附着和招募的数量更多，在八月幼虫在高润湿性的载玻片上附着和招募的数量也更多。珍珠港生物膜群落在一系列表面润湿性上的发展相似，并且在珍珠港放置10天后，所有表面对优美盘管虫幼虫的吸引力相同，无论初始表面特性如何。
