Photocrosslinking of RNA and photoMet-containing amphiphilic alpha-helical peptides.
作者信息
Hyun Soonsil, Han Areum, Yu Jaehoon
机构信息
Department of Chemistry and Education, Seoul National University, Korea.
出版信息
Chembiochem. 2009 Apr 17;10(6):987-9. doi: 10.1002/cbic.200900100.
PMID:19308928
Abstract
Discovering RNA-protein interactions: A library of photoMet-containing peptides was synthesized by using an Arg- and Leu-rich alpha-helical amphiphilic peptide. Irradiation of mixtures of these peptides and Rev-responsive element (RRE) hairpin RNA promoted formation of covalent adducts. Analysis of one adduct showed that U26 in the bulged stem is responsible for covalent bond formation with the carbene intermediate. This strategy can provide important structural information about RNA-peptide interactions.
摘要
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