Pinna G G, Spano A, Carru C, Pes G M, Delogu G, Miscali A R, Zanetti S, Sisini A
Istituto di Chimica Biologica, Facoltà di Medicina e Chirurgia, Università di Sassari.
Boll Soc Ital Biol Sper. 1991 Mar;67(3):287-94.
Pseudomonas aeruginosa is a microorganism of the terrestrial and aquatic environment which may cause pulmonary, urinary and corneal infections. The pathogenesis of Pseudomonas-induced diseases seems to be linked to the production of extracellular substances such as exotoxins, hemolysins, leukocidins and hydrolytic enzymes, including various peptidases. Anyway there is not a clear view on the role other proteases have in the mechanism of pathogenesis, and even if their activity may always be associated to the toxicity of the microorganism. We have therefore determined the activity of a number of eso- and endopeptidases in 17 strains of P. aeruginosa isolated from patients with urinary pathologies. Four of these peptidase activities, namely elastase, neutral protease, aminopeptidase I and leucine aminopeptidase show a positive correlation with three parameters selected as indices of toxicity, i.e. the mucoid appearance, the gentamicin resistance and the adhesivity of colonies.
铜绿假单胞菌是一种存在于陆地和水生环境中的微生物，可引起肺部、泌尿系统和角膜感染。铜绿假单胞菌所致疾病的发病机制似乎与细胞外物质的产生有关，如外毒素、溶血素、杀白细胞素和水解酶，包括各种肽酶。然而，对于其他蛋白酶在发病机制中的作用尚无明确认识，即便它们的活性可能总是与微生物的毒性相关。因此，我们测定了从泌尿系统疾病患者中分离出的17株铜绿假单胞菌中多种胞外和胞内肽酶的活性。其中四种肽酶活性，即弹性蛋白酶、中性蛋白酶、氨肽酶I和亮氨酸氨肽酶，与选定为毒性指标的三个参数呈正相关，这三个参数分别是黏液样外观、庆大霉素耐药性和菌落黏附性。
