可变剪接途径的PCR分析：异源双链体形成产生的假象的鉴定 - Suppr

Suppr

超能文献

PCR analysis of alternative splicing pathways: identification of artifacts generated by heteroduplex formation.

作者信息

Zorn A M, Krieg P A

机构信息

Department of Zoology, University of Texas, Austin 78712.

出版信息

Biotechniques. 1991 Aug;11(2):180, 182, 184.

PMID:1931014

Abstract

摘要

相似文献

PCR analysis of alternative splicing pathways: identification of artifacts generated by heteroduplex formation.可变剪接途径的PCR分析：异源双链体形成产生的假象的鉴定

Biotechniques. 1991 Aug;11(2):180, 182, 184.

Discriminating PCR artifacts using directed heteroduplex analysis (DHDA).使用定向异源双链分析（DHDA）鉴别聚合酶链式反应（PCR）产物。

Biotechniques. 1995 Dec;19(6):902-5.

How to correct for errors in mRNA quantitation by competitive PCR due to heteroduplex formation of amplification products.

Cell Mol Biol (Noisy-le-grand). 1997 Sep;43(6):841-50.

Limitations imposed by heteroduplex formation on quantitative RT-PCR.异源双链体形成对定量逆转录聚合酶链反应的限制

Biochem Biophys Res Commun. 1996 Sep 4;226(1):113-7. doi: 10.1006/bbrc.1996.1319.

Seminested polymerase chain reaction and heteroduplex analysis detects the monoclonality of IgH rearrangement in follicular lymphoma patients with high sensitivity.半巢式聚合酶链反应和异源双链分析能高度灵敏地检测滤泡性淋巴瘤患者免疫球蛋白重链（IgH）重排的单克隆性。

Haematologica. 2005 Feb;90(2):272-3.

Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'.混合模板扩增中的异源双链体：形成、后果及通过“修复PCR”消除

Nucleic Acids Res. 2002 May 1;30(9):2083-8. doi: 10.1093/nar/30.9.2083.

Heteroduplex resolution using T7 endonuclease I in microbial community analyses.在微生物群落分析中使用T7核酸内切酶I进行异源双链体解析。

Biotechniques. 2000 Apr;28(4):676-8, 680, 681. doi: 10.2144/00284st03.

Detection and quantitation of heteroduplexes can increase the reliability in competitive polymerase chain reaction.异源双链体的检测和定量可以提高竞争性聚合酶链反应的可靠性。

Anal Biochem. 1999 Mar 15;268(2):398-401. doi: 10.1006/abio.1998.3049.

Subtype-specific polymerase chain reaction for the identification of HIV-1 genetic subtypes circulating in Africa.用于鉴定在非洲流行的HIV-1基因亚型的亚型特异性聚合酶链反应

AIDS. 1998 Apr 16;12(6):671-3.

Detection of two point mutations causing familial defective apolipoprotein B-100 by heteroduplex analysis.

Mol Cell Probes. 1994 Dec;8(6):513-8. doi: 10.1006/mcpr.1994.1072.

引用本文的文献

Mhc class II diversity and balancing selection in greater prairie-chickens.大草原榛鸡的MHC II类多样性与平衡选择

Genetica. 2010 Feb;138(2):265-71. doi: 10.1007/s10709-009-9417-4. Epub 2009 Oct 23.

Single locus typing of MHC class I and class II B loci in a population of red jungle fowl.红原鸡群体中MHC I类和II类B基因座的单基因座分型

Immunogenetics. 2008 May;60(5):233-47. doi: 10.1007/s00251-008-0288-0. Epub 2008 Apr 4.

Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'.混合模板扩增中的异源双链体：形成、后果及通过“修复PCR”消除

Nucleic Acids Res. 2002 May 1;30(9):2083-8. doi: 10.1093/nar/30.9.2083.

DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.微生物的DNA指纹技术。分类与命名建议

Mol Biotechnol. 1996 Oct;6(2):115-42. doi: 10.1007/BF02740768.

A delta T-cell receptor deleting element transgenic reporter construct is rearranged in alpha beta but not gamma delta T-cell lineages.一种δ T细胞受体缺失元件转基因报告构建体在αβ而非γδ T细胞谱系中发生重排。

Mol Cell Biol. 1995 Dec;15(12):7022-31. doi: 10.1128/MCB.15.12.7022.

Analysis of multiple mRNAs from pathogenic equine infectious anemia virus (EIAV) in an acutely infected horse reveals a novel protein, Ttm, derived from the carboxy terminus of the EIAV transmembrane protein.对一匹急性感染马体内致病性马传染性贫血病毒（EIAV）的多种mRNA进行分析，发现了一种新蛋白Ttm，它源自EIAV跨膜蛋白的羧基末端。

J Virol. 1993 Feb;67(2):832-42. doi: 10.1128/JVI.67.2.832-842.1993.

Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site.秀丽隐杆线虫中的剪接在3'剪接受体位点不需要AG。

Mol Cell Biol. 1993 Jan;13(1):626-37. doi: 10.1128/mcb.13.1.626-637.1993.

Deletion of 19 base pairs in intron 13 of the gene for the pro alpha 2(I) chain of type-I procollagen (COL1A2) causes exon skipping in a proband with type-I osteogenesis imperfecta.

Hum Genet. 1993 Apr;91(3):210-6. doi: 10.1007/BF00218258.

Alternative splicing of the mouse amelogenin primary RNA transcript.小鼠釉原蛋白初级RNA转录本的可变剪接

Calcif Tissue Int. 1994 Oct;55(4):302-10. doi: 10.1007/BF00310410.

Two unusual forms of human immunoglobulin E encoded by alternative RNA splicing of epsilon heavy chain membrane exons.由ε重链膜外显子的可变RNA剪接编码的两种异常形式的人类免疫球蛋白E

J Exp Med. 1992 Jul 1;176(1):233-43. doi: 10.1084/jem.176.1.233.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验