Vaneechoutte M
Department of Clinical Chemistry, Microbiology & Immunology Blok A, University Hospital, Ghent, Belgium.
Mol Biotechnol. 1996 Oct;6(2):115-42. doi: 10.1007/BF02740768.
A whole array of DNA-fingerprinting techniques, which provide indirect access to DNA sequence polymorphism in order to assess species or clonal identity of bacterial organisms or in order to study bacterial genome composition, have been described during past decades. Nomenclature has been sometimes erroneous and/or confusing, also because of hybrid techniques that combine different approaches. It can be shown that most techniques study the sequence polymorphism of only the chromosome, or only the plasmid(s) or only a gene or gene fragment and that the sequence polymorphism is revealed by AFLP (amplified fragment length polymorphism) or by RFLP (restriction fragment length polymorphism) or by special electrophoresis techniques. Starting from these considerations, some taxonomy of techniques, which enables more appropriate nomenclature, can be developed.
在过去几十年间，已经描述了一系列DNA指纹技术，这些技术通过间接获取DNA序列多态性来评估细菌生物的物种或克隆身份，或者研究细菌基因组组成。由于结合了不同方法的杂交技术，命名有时会出现错误和/或混淆。可以表明，大多数技术仅研究染色体、或仅研究质粒、或仅研究一个基因或基因片段的序列多态性，并且序列多态性通过扩增片段长度多态性（AFLP）、或限制性片段长度多态性（RFLP）、或特殊电泳技术得以揭示。基于这些考虑，可以开发一些技术分类法，从而实现更恰当的命名。
