Shaha C, Seshadri T, Suri A, Talwar G P
Sperm Biotechnology Laboratory, National Institute of Immunology, New Delhi, India.
Mol Reprod Dev. 1991 Jul;29(3):302-11. doi: 10.1002/mrd.1080290314.
A group of antigens of 24-kD Mr from rat testes were characterised biochemically. These antigens were part of a larger molecule of approximately 200 kD. On treatment with disulfide bond reducing agent, the 200-kD molecule was reduced to subunits. Immunoreactivity was confined to a doublet of approximately 24 kD and a single band of approximately 50 kD Mr after the reduction. Glycoprotein in nature, this antigen shared immunoreactive epitopes with a 40-kD antigen on human spermatozoa. Antiserum raised in rabbits against the 24-kD antigen from rat testes reacted with antigens on the acrosome of human spermatozoa. Agglutination of sperm could be induced by the antiserum. The carbohydrate residue could be removed by mannosidase digestion. Chemical deglycosylation studies showed a slight decrease in molecular weight. Immunoreactivity was however not completely lost after chemical deglycosylation. Isoelectric focusing of the antigen identified nine isoelectric species. Two relatively minor species showed immunoreactivity. Acrosome-reacted spermatozoa showed loss of antigens from acrosome.
对来自大鼠睾丸的一组分子量为24kD的抗原进行了生化特性分析。这些抗原是一个约200kD的较大分子的一部分。用二硫键还原剂处理后，200kD的分子被还原为亚基。还原后，免疫反应性局限于一条约24kD的双条带和一条约50kD的单条带。该抗原本质上是糖蛋白，与人类精子上的一种40kD抗原具有共同的免疫反应性表位。用大鼠睾丸的24kD抗原免疫兔产生的抗血清与人类精子顶体上的抗原发生反应。该抗血清可诱导精子凝集。通过甘露糖苷酶消化可去除碳水化合物残基。化学去糖基化研究表明分子量略有降低。然而，化学去糖基化后免疫反应性并未完全丧失。该抗原的等电聚焦鉴定出9种等电形式。两种相对较少的形式表现出免疫反应性。顶体反应的精子显示顶体抗原丢失。
