通过深度RACE技术对转录起始位点进行高通量验证。

High-throughput verification of transcriptional starting sites by Deep-RACE.

作者信息

Olivarius Signe, Plessy Charles, Carninci Piero

机构信息

Functional Genomics Technology Team, Omics Science Center, RIKEN Yokohama Institute, Kanagawa, Japan.

出版信息

Biotechniques. 2009 Feb;46(2):130-2. doi: 10.2144/000113066.

DOI:10.2144/000113066

PMID:19317658

Abstract

We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes and is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.

摘要

我们提出了一种用于研究感兴趣基因转录起始位点的高通量方法，我们将其命名为Deep-RACE（深度cDNA末端快速扩增）。利用最新的测序技术，它能够对多个基因进行平行分析，并且无需耗时的克隆步骤。与RACE PCR产物测序相比，即使对于小至17个样本的批次，我们的方法也更精确且更具成本效益。