Kamilya Tapanendu, Pal Prabir, Mahato Mrityunjoy, Talapatra G B
Department of Spectroscopy, Indian Association for the Cultivation of Science, Jadavpur, Kolkata-700 032, India.
J Phys Chem B. 2009 Apr 16;113(15):5128-35. doi: 10.1021/jp9001059.
We report here the effect of salt (KCl) on the interfacial surface activity of yeast alcohol dehydogenease (ADH) at air/water interface using the Langmuir-Blodgett technique. Effect of salt content in the water subphase on ADH structure has been studied. The change of area/molecule, compressibility, rigidity, and unfolding of ADH are insignificant up to 10 mM KCl concentration. The significant changes are observed above 0.1 M KCl concentrations. Observations are explained in the context of DLVO theory. FTIR study of amide band together with AFM imaging of ADH monolayer indicate that KCl perturbs the ADH monolayer by the increment of beta-structure resulting into larger unfolding and intermolecular aggregates at high salt concentration.
我们在此报告使用朗缪尔-布洛杰特技术研究盐（KCl）对酵母乙醇脱氢酶（ADH）在空气/水界面的界面表面活性的影响。已研究水亚相中盐含量对ADH结构的影响。在KCl浓度高达10 mM时，ADH的分子面积、压缩性、刚性和展开情况的变化不显著。在KCl浓度高于0.1 M时观察到显著变化。在DLVO理论的背景下对观察结果进行了解释。对酰胺带的傅里叶变换红外光谱研究以及ADH单层的原子力显微镜成像表明，KCl通过增加β-结构扰乱ADH单层，导致在高盐浓度下出现更大程度的展开和分子间聚集体。
