来自不同十二指肠贾第虫组合的贾第虫病毒（GLV）的检测与比较。

Detection and comparison of Giardia virus (GLV) from different assemblages of Giardia duodenalis.

作者信息

Miska K B, Jenkins M C, Trout J M, Santín M, Fayer R

机构信息

USDA/ARS, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705, USA.

出版信息

J Parasitol. 2009 Oct;95(5):1197-200. doi: 10.1645/GE-1876.1.

DOI:10.1645/GE-1876.1

PMID:19320541

Abstract

Five assemblages of Giardia duodenalis were identified from cysts in cattle, dog, cat, sheep, and reindeer feces using ribosomal DNA (rDNA) sequencing. Assemblage A was present in cattle and reindeer feces, Assemblages C and D were present in dog feces, Assemblage E was present in cattle and sheep feces, and Assemblage F was present in cat feces. Giardia virus, originally referred to as Giardia lamblia virus (GLV), is a double-stranded RNA virus. Primers designed for the GLV capsid protein gene identified GLV sequences in G. lamblia from a reindeer (Assemblage A) and from a dog (Assemblage C). Two distinct GLV sequences were identified in the dog specimen and 1 sequence was identified in the reindeer specimen. None of these GLV sequences was identical with previously published GLV sequences. It appears that GLVs are genetically diverse and that more than 1 virion can be present in a single sample. Because many of the specimens that contained cysts were found to be negative for GLV, it appears that this test for capsid protein is of limited value for the purposes of detecting G. lamblia.

摘要

利用核糖体DNA（rDNA）测序技术，从牛、狗、猫、绵羊和驯鹿粪便中的囊肿中鉴定出了五种十二指肠贾第虫组合。组合A存在于牛和驯鹿粪便中，组合C和D存在于狗粪便中，组合E存在于牛和绵羊粪便中，组合F存在于猫粪便中。贾第虫病毒最初被称为蓝氏贾第鞭毛虫病毒（GLV），是一种双链RNA病毒。针对GLV衣壳蛋白基因设计的引物在来自驯鹿（组合A）和狗（组合C）的蓝氏贾第鞭毛虫中鉴定出了GLV序列。在狗的样本中鉴定出了两种不同的GLV序列，在驯鹿样本中鉴定出了1种序列。这些GLV序列均与先前发表的GLV序列不同。看来GLV在基因上具有多样性，并且单个样本中可能存在不止一种病毒粒子。由于许多含有囊肿的样本被发现GLV呈阴性，因此这种衣壳蛋白检测对于检测蓝氏贾第鞭毛虫的目的似乎价值有限。