• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Stimulatory heterotrimeric GTP-binding protein augments cisplatin-induced apoptosis by upregulating Bak expression in human lung cancer cells.刺激性异源三聚体GTP结合蛋白通过上调人肺癌细胞中Bak的表达增强顺铂诱导的细胞凋亡。
Cancer Sci. 2009 Jun;100(6):1069-74. doi: 10.1111/j.1349-7006.2009.01136.x. Epub 2009 Mar 23.
2
Stimulatory heterotrimeric G protein augments gamma ray-induced apoptosis by up-regulation of Bak expression via CREB and AP-1 in H1299 human lung cancer cells.刺激性异源三聚体G蛋白通过在H1299人肺癌细胞中经由CREB和AP-1上调Bak表达来增强γ射线诱导的细胞凋亡。
Exp Mol Med. 2009 Aug 31;41(8):592-600. doi: 10.3858/emm.2009.41.8.065.
3
Cisplatin-induced apoptosis in non-small-cell lung cancer cells is dependent on Bax- and Bak-induction pathway and synergistically activated by BH3-mimetic ABT-263 in p53 wild-type and mutant cells.顺铂诱导的非小细胞肺癌细胞凋亡依赖于Bax和Bak诱导途径，并在p53野生型和突变型细胞中被BH3模拟物ABT-263协同激活。
Biochem Biophys Res Commun. 2016 Apr 29;473(2):490-6. doi: 10.1016/j.bbrc.2016.03.053. Epub 2016 Mar 18.
4
Heterotrimeric stimulatory GTP-binding proteins inhibit cisplatin-induced apoptosis by increasing X-linked inhibitor of apoptosis protein expression in cervical cancer cells.三聚体刺激 GTP 结合蛋白通过增加宫颈癌细胞中 X 连锁凋亡抑制蛋白的表达来抑制顺铂诱导的细胞凋亡。
Cancer Sci. 2011 Apr;102(4):837-44. doi: 10.1111/j.1349-7006.2011.01883.x. Epub 2011 Feb 28.
5
Stimulatory heterotrimeric GTP-binding protein inhibits hydrogen peroxide-induced apoptosis by repressing BAK induction in SH-SY5Y human neuroblastoma cells.刺激性异源三聚体GTP结合蛋白通过抑制SH-SY5Y人神经母细胞瘤细胞中BAK的诱导来抑制过氧化氢诱导的细胞凋亡。
J Biol Chem. 2008 Jan 18;283(3):1350-1361. doi: 10.1074/jbc.M702344200. Epub 2007 Nov 8.
6
cAMP signaling inhibits radiation-induced ATM phosphorylation leading to the augmentation of apoptosis in human lung cancer cells.环磷酸腺苷（cAMP）信号传导抑制辐射诱导的共济失调毛细血管扩张突变基因（ATM）磷酸化，导致人肺癌细胞凋亡增加。
Mol Cancer. 2014 Feb 24;13:36. doi: 10.1186/1476-4598-13-36.
7
Erlotinib induces mitochondrial-mediated apoptosis in human H3255 non-small-cell lung cancer cells with epidermal growth factor receptorL858R mutation through mitochondrial oxidative phosphorylation-dependent activation of BAX and BAK.厄洛替尼通过线粒体氧化磷酸化依赖性激活BAX和BAK，诱导具有表皮生长因子受体L858R突变的人H3255非小细胞肺癌细胞发生线粒体介导的凋亡。
Mol Pharmacol. 2008 Sep;74(3):793-806. doi: 10.1124/mol.107.044396. Epub 2008 Jun 4.
8
Cyclic GMP/protein kinase G type-Iα (PKG-Iα) signaling pathway promotes CREB phosphorylation and maintains higher c-IAP1, livin, survivin, and Mcl-1 expression and the inhibition of PKG-Iα kinase activity synergizes with cisplatin in non-small cell lung cancer cells.环鸟苷酸/蛋白激酶 G 型-Iα (PKG-Iα) 信号通路促进 CREB 磷酸化，维持较高的 c-IAP1、livin、survivin 和 Mcl-1 的表达，PKG-Iα 激酶活性的抑制与顺铂在非小细胞肺癌细胞中具有协同作用。
J Cell Biochem. 2012 Nov;113(11):3587-98. doi: 10.1002/jcb.24237.
9
Inhibition of gamma ray-induced apoptosis by stimulatory heterotrimeric GTP binding protein involves Bcl-xL down-regulation in SH-SY5Y human neuroblastoma cells.刺激性异三聚体GTP结合蛋白对γ射线诱导的细胞凋亡的抑制作用涉及SH-SY5Y人神经母细胞瘤细胞中Bcl-xL的下调。
Exp Mol Med. 2007 Oct 31;39(5):583-93. doi: 10.1038/emm.2007.64.
10
Activation of Bak and Bax through c-abl-protein kinase Cdelta-p38 MAPK signaling in response to ionizing radiation in human non-small cell lung cancer cells.在人非小细胞肺癌细胞中，通过c-abl-蛋白激酶Cδ-p38丝裂原活化蛋白激酶信号传导途径激活Bak和Bax以响应电离辐射。
J Biol Chem. 2006 Mar 17;281(11):7049-59. doi: 10.1074/jbc.M512000200. Epub 2006 Jan 12.

引用本文的文献

1
Upregulation of HOXA13 as a potential tumorigenesis and progression promoter of LUSC based on qRT-PCR and bioinformatics.基于qRT-PCR和生物信息学分析，HOXA13上调作为肺鳞状细胞癌潜在的肿瘤发生和进展促进因子
Int J Clin Exp Pathol. 2017 Oct 1;10(10):10650-10665. eCollection 2017.
2
cAMP signaling increases histone deacetylase 8 expression via the Epac2-Rap1A-Akt pathway in H1299 lung cancer cells.在H1299肺癌细胞中，环磷酸腺苷（cAMP）信号通过Epac2-Rap1A-Akt途径增加组蛋白去乙酰化酶8的表达。
Exp Mol Med. 2017 Feb 24;49(2):e297. doi: 10.1038/emm.2016.152.
3
Cyclic AMP signaling reduces sirtuin 6 expression in non-small cell lung cancer cells by promoting ubiquitin-proteasomal degradation via inhibition of the Raf-MEK-ERK (Raf/mitogen-activated extracellular signal-regulated kinase/extracellular signal-regulated kinase) pathway.环磷酸腺苷（cAMP）信号传导通过抑制Raf-丝裂原活化细胞外信号调节激酶/细胞外信号调节激酶（Raf-MEK-ERK）途径促进泛素-蛋白酶体降解，从而降低非小细胞肺癌细胞中沉默调节蛋白6（sirtuin 6）的表达。
J Biol Chem. 2015 Apr 10;290(15):9604-13. doi: 10.1074/jbc.M114.633198. Epub 2015 Feb 24.
4
Gαs protein expression is an independent predictor of recurrence in prostate cancer.G 蛋白表达是前列腺癌复发的独立预测因子。
J Immunol Res. 2014;2014:301376. doi: 10.1155/2014/301376. Epub 2014 Mar 31.
5
cAMP signaling inhibits radiation-induced ATM phosphorylation leading to the augmentation of apoptosis in human lung cancer cells.环磷酸腺苷（cAMP）信号传导抑制辐射诱导的共济失调毛细血管扩张突变基因（ATM）磷酸化，导致人肺癌细胞凋亡增加。
Mol Cancer. 2014 Feb 24;13:36. doi: 10.1186/1476-4598-13-36.
6
Heterotrimeric G proteins and apoptosis: intersecting signaling pathways leading to context dependent phenotypes.异三聚体 G 蛋白与细胞凋亡：相互交汇的信号通路导致与背景相关的表型。
Curr Mol Med. 2009 Jun;9(5):527-45. doi: 10.2174/156652409788488784.

本文引用的文献

1
Growth suppression of lung cancer cells by targeting cyclic AMP response element-binding protein.通过靶向环磷酸腺苷反应元件结合蛋白抑制肺癌细胞生长
Cancer Res. 2008 Feb 15;68(4):981-8. doi: 10.1158/0008-5472.CAN-06-0249.
2
Inhibition of gamma ray-induced apoptosis by stimulatory heterotrimeric GTP binding protein involves Bcl-xL down-regulation in SH-SY5Y human neuroblastoma cells.刺激性异三聚体GTP结合蛋白对γ射线诱导的细胞凋亡的抑制作用涉及SH-SY5Y人神经母细胞瘤细胞中Bcl-xL的下调。
Exp Mol Med. 2007 Oct 31;39(5):583-93. doi: 10.1038/emm.2007.64.
3
Stimulatory heterotrimeric GTP-binding protein inhibits hydrogen peroxide-induced apoptosis by repressing BAK induction in SH-SY5Y human neuroblastoma cells.刺激性异源三聚体GTP结合蛋白通过抑制SH-SY5Y人神经母细胞瘤细胞中BAK的诱导来抑制过氧化氢诱导的细胞凋亡。
J Biol Chem. 2008 Jan 18;283(3):1350-1361. doi: 10.1074/jbc.M702344200. Epub 2007 Nov 8.
4
Peroxide is a key mediator of Bcl-2 down-regulation and apoptosis induction by cisplatin in human lung cancer cells.过氧化物是顺铂在人肺癌细胞中下调Bcl-2和诱导凋亡的关键介质。
Mol Pharmacol. 2008 Jan;73(1):119-27. doi: 10.1124/mol.107.040873. Epub 2007 Oct 2.
5
CREB--a real culprit in oncogenesis.CREB——肿瘤发生中的真正罪魁祸首。
FEBS J. 2007 Jul;274(13):3224-32. doi: 10.1111/j.1742-4658.2007.05884.x. Epub 2007 Jun 12.
6
Molecular mechanisms of resistance and toxicity associated with platinating agents.与铂类药物相关的耐药性和毒性的分子机制。
Cancer Treat Rev. 2007 Feb;33(1):9-23. doi: 10.1016/j.ctrv.2006.09.006. Epub 2006 Nov 3.
7
Treatment of advanced non-small cell lung cancer: chemotherapy with or without cisplatin?晚期非小细胞肺癌的治疗：含或不含顺铂的化疗？
Ann Oncol. 2006 Mar;17 Suppl 2:ii83-87. doi: 10.1093/annonc/mdj933.
8
Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues.人类组织中cAMP反应元件结合蛋白占据、磷酸化及靶基因激活的全基因组分析。
Proc Natl Acad Sci U S A. 2005 Mar 22;102(12):4459-64. doi: 10.1073/pnas.0501076102. Epub 2005 Mar 7.
9
G-protein signaling: back to the future.G蛋白信号传导：回归未来。
Cell Mol Life Sci. 2005 Mar;62(5):551-77. doi: 10.1007/s00018-004-4462-3.
10
The role of reactive oxygen species in cisplatin-induced apoptosis in human malignant testicular germ cell lines.活性氧在顺铂诱导人恶性睾丸生殖细胞系凋亡中的作用。
Int J Oncol. 2004 Dec;25(6):1671-6. doi: 10.3892/ijo.25.6.1671.

刺激性异源三聚体GTP结合蛋白通过上调人肺癌细胞中Bak的表达增强顺铂诱导的细胞凋亡。

Stimulatory heterotrimeric GTP-binding protein augments cisplatin-induced apoptosis by upregulating Bak expression in human lung cancer cells.

作者信息

Choi Yoon Jung, Oh Jung-Min, Kim So-Young, Seo Miran, Juhnn Yong-Sung

机构信息

Department of Biochemistry and Molecular Biology, Laboratory of Cellular Signaling, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.

出版信息

Cancer Sci. 2009 Jun;100(6):1069-74. doi: 10.1111/j.1349-7006.2009.01136.x. Epub 2009 Mar 23.

DOI:10.1111/j.1349-7006.2009.01136.x
PMID:19320642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11158464/
Abstract

The present study aimed to investigate the effect of the stimulatory heterotrimeric GTP-binding (Gs) protein signaling system on cisplatin-induced apoptosis of lung cancer cells and its underlying mechanism as an attempt to develop a novel strategy to improve the therapeutic efficacy of cisplatin. Overexpression of the constitutively active alpha subunit of Gs (GalphasQL) in A549 human lung cancer cells increased cisplatin-induced apoptosis, and knockdown of Galphas with small hairpin RNA decreased the percentage of apoptotic cells. GalphasQL increased the expression of the proapoptotic proteins B-cell leukemia/lymphoma-2 genes (Bcl-2) homologous antagonist killer protein (Bak) and Bcl-2 associated X protein (Bax), and decreased the expression of the antiapoptotic proteins Bcl-2 and Bcl-Xlong protein. Knockdown of Bak blocked the augmentative effects of GalphasQL. GalphasQL decreased the degradation rate of the Bak protein, and increased Bak mRNA transcript levels. GalphasQL increased Bak-luciferase activity in a protein kinase A and cyclic AMP response element-dependent manner. GalphasQL also augmented cisplatin-induced apoptosis of H1299 human lung cancer cells that lack functional p53. From this study, it is concluded that Galphas augments cisplatin-induced apoptosis of lung cancer cells partially through upregulating Bak expression by increasing transcription and by decreasing the rate of protein degradation.

摘要

本研究旨在探讨刺激性异三聚体GTP结合（Gs）蛋白信号系统对顺铂诱导肺癌细胞凋亡的影响及其潜在机制，试图开发一种提高顺铂治疗疗效的新策略。在A549人肺癌细胞中过表达组成型活性α亚基Gs（GalphasQL）可增加顺铂诱导的细胞凋亡，而用小发夹RNA敲低Galphas可降低凋亡细胞的百分比。GalphasQL增加促凋亡蛋白B细胞白血病/淋巴瘤-2基因（Bcl-2）同源拮抗剂杀伤蛋白（Bak）和Bcl-2相关X蛋白（Bax）的表达，并降低抗凋亡蛋白Bcl-2和Bcl-Xlong蛋白的表达。敲低Bak可阻断GalphasQL的增强作用。GalphasQL降低了Bak蛋白的降解率，并增加了Bak mRNA转录水平。GalphasQL以蛋白激酶A和环磷酸腺苷反应元件依赖的方式增加Bak荧光素酶活性。GalphasQL还增强了顺铂对缺乏功能性p53的H1299人肺癌细胞的诱导凋亡作用。从本研究得出结论，Galphas通过增加转录和降低蛋白降解率来上调Bak表达，从而部分增强顺铂诱导的肺癌细胞凋亡。