Dugelay S, Baverel G
Laboratoire de Physiologie Rénale et Métabolique, CNRS URA 1177 et INSERM U 80, Lyon, France.
Biochim Biophys Acta. 1991 Oct 10;1075(2):191-4. doi: 10.1016/0304-4165(91)90251-b.
When rabbit kidney tubules were incubated with 1 mM [1-14C]glutamine as substrate, a release of 14CO2 together with a net production of glutamine were observed. That glutamine utilization was masked by higher rates of concomitant glutamine synthesis was demonstrated by: (i) inhibiting glutamine synthesis; and (ii) measuring the specific radioactivity of [1-14C]glutamine which fell during incubation.
当兔肾小管与1 mM [1-¹⁴C]谷氨酰胺作为底物一起孵育时，观察到¹⁴CO₂的释放以及谷氨酰胺的净生成。谷氨酰胺的利用被同时发生的更高谷氨酰胺合成速率所掩盖，这通过以下方式得到证明：(i) 抑制谷氨酰胺合成；以及 (ii) 测量孵育期间下降的 [1-¹⁴C]谷氨酰胺的比放射性。
