一种绦虫——鹌鹑新圆线虫的鞘脂类

Sphingolipids of a cestode Metroliasthes coturnix.

作者信息

Nishimura K, Suzuki A, Kino H

机构信息

Department of Biology, Keio University, Medical School, Yokohama, Japan.

出版信息

Biochim Biophys Acta. 1991 Nov 5;1086(2):141-50. doi: 10.1016/0005-2760(91)90001-x.

DOI:10.1016/0005-2760(91)90001-x

PMID:1932097

Abstract

Sphingolipids of Metroliasthes coturnix were studied. The cestode contained no detectable amounts of sphingomyelin. The major glycosphingolipids found were monogalactosylceramide, galactosyl-alpha-1-4-galactosylceramide, galactosyl-beta-1-6-galactosyl-beta-1-6-galactosylceramide and galactosyl-beta-1-6-galactosyl-beta-1-6-galactosyl-beta-1-6-galactosylce ramide. Their ceramides were mostly composed of C18-20 sphinganine or 4-D-hydroxysphinganine, which is N-acylated by extraordinarily long normal and 2-hydroxy acids such as C26 acid (range C16 to C30) except tetragalactosylceramide. The structure of glycosphingolipids was confirmed after their chromatographic separation into each molecular species using a novel analytical device, fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography.

摘要

对鹌鹑毛细线虫的鞘脂进行了研究。该绦虫未检测到鞘磷脂。发现的主要糖鞘脂有单半乳糖基神经酰胺、半乳糖基-α-1-4-半乳糖基神经酰胺、半乳糖基-β-1-6-半乳糖基-β-1-6-半乳糖基神经酰胺和半乳糖基-β-1-6-半乳糖基-β-1-6-半乳糖基-β-1-6-半乳糖基神经酰胺。除四半乳糖基神经酰胺外，它们的神经酰胺大多由C18 - 20鞘氨醇或4-D-羟基鞘氨醇组成，这些鞘氨醇被极长的正常酸和2-羟基酸（如C26酸，范围为C16至C30）N-酰化。使用一种新型分析装置——与反相高效液相色谱联用的快原子轰击质谱仪，将糖鞘脂色谱分离成各个分子种类后，确认了其结构。