Suppr超能文献

集胞藻PCC 6803膜蛋白的鉴定及生物信息学分析

Identification and bioinformatic analysis of the membrane proteins of synechocystis sp. PCC 6803.

作者信息

Wang Yingchun, Xu Wu, Chitnis Parag R

机构信息

Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, 50011, USA.

出版信息

Proteome Sci. 2009 Mar 25;7:11. doi: 10.1186/1477-5956-7-11.

Abstract

BACKGROUND

The membranes of Synechocystis sp. PCC 6803 play a central role in photosynthesis, respiration and other important metabolic pathways. Comprehensive identification of the membrane proteins is of importance for a better understanding of the diverse functions of its unique membrane structures. Up to date, approximately 900 known or predicted membrane proteins, consisting 24.5% of Synechocystis sp. PCC 6803 proteome, have been indentified by large-scale proteomic studies.

RESULTS

To resolve more membrane proteins on 2-D gels for mass spectrometry identification, we separated integral proteins from membrane associated proteins and collected them as the integral and peripheral fractions, respectively. In total, 95 proteins in the peripheral fraction and 29 proteins in the integral fraction were identified, including the 5 unique proteins that were not identified by any previous studies. Bioinformatic analysis revealed that the identified proteins can be functionally classified into 14 distinct groups according to the cellular functions annotated by Cyanobase, including the two largest groups hypothetical and unknown, and photosynthesis and respiration. Homology analysis indicates that the identified membrane proteins are more conserved than the rest of the proteome.

CONCLUSION

The proteins identified in this study combined with other published proteomic data provide the most comprehensive Synechocystis proteome catalog, which will serve as a useful reference for further detailed studies to address protein functions through both traditional gene-by-gene and systems biology approaches.

摘要

背景

集胞藻PCC 6803的膜在光合作用、呼吸作用及其他重要代谢途径中发挥核心作用。全面鉴定膜蛋白对于更好地理解其独特膜结构的多样功能至关重要。到目前为止,通过大规模蛋白质组学研究已鉴定出约900种已知或预测的膜蛋白,占集胞藻PCC 6803蛋白质组的24.5%。

结果

为了在二维凝胶上分离更多膜蛋白用于质谱鉴定,我们将整合蛋白与膜相关蛋白分离,并分别将它们收集为整合部分和外周部分。总共鉴定出外周部分中的95种蛋白和整合部分中的29种蛋白,包括5种以前任何研究都未鉴定出的独特蛋白。生物信息学分析表明,根据蓝藻数据库注释的细胞功能,鉴定出的蛋白可在功能上分为14个不同的组,包括最大的两组,即假定的和未知的,以及光合作用和呼吸作用相关的组。同源性分析表明,鉴定出的膜蛋白比蛋白质组的其他部分更保守。

结论

本研究中鉴定出的蛋白与其他已发表的蛋白质组学数据相结合,提供了最全面的集胞藻蛋白质组目录,这将为通过传统的逐个基因和系统生物学方法进一步详细研究蛋白功能提供有用的参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dad1/2666656/451a10ec541d/1477-5956-7-11-1.jpg

相似文献

2
Proteomic studies of the thylakoid membrane of Synechocystis sp. PCC 6803.
Proteomics. 2005 Dec;5(18):4905-16. doi: 10.1002/pmic.200500111.
3
Isolation of outer membrane of Synechocystis sp. PCC 6803 and its proteomic characterization.
Mol Cell Proteomics. 2004 Jun;3(6):586-95. doi: 10.1074/mcp.M300137-MCP200. Epub 2004 Feb 26.
4
Proteomics of Synechocystis sp. PCC 6803. Identification of novel integral plasma membrane proteins.
FEBS J. 2007 Feb;274(3):791-804. doi: 10.1111/j.1742-4658.2006.05624.x.
5
Proteomic study of the peripheral proteins from thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803.
Electrophoresis. 2000 May;21(9):1746-54. doi: 10.1002/(SICI)1522-2683(20000501)21:9<1746::AID-ELPS1746>3.0.CO;2-O.
6
Identification of the proteomic changes in Synechocystis sp. PCC 6803 following prolonged UV-B irradiation.
J Exp Bot. 2009;60(4):1141-54. doi: 10.1093/jxb/ern356. Epub 2009 Mar 4.
10
Proteomic study of the impact of Hik33 mutation in Synechocystis sp. PCC 6803 under normal and salt stress conditions.
J Proteome Res. 2012 Jan 1;11(1):502-14. doi: 10.1021/pr200811s. Epub 2011 Nov 16.

引用本文的文献

3
Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium.
Mol Cell Proteomics. 2022 Dec;21(12):100440. doi: 10.1016/j.mcpro.2022.100440. Epub 2022 Nov 8.
4
Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism.
Plant Physiol. 2019 Dec;181(4):1721-1738. doi: 10.1104/pp.19.00897. Epub 2019 Oct 2.
6
Global Proteomic Analysis Reveals an Exclusive Role of Thylakoid Membranes in Bioenergetics of a Model Cyanobacterium.
Mol Cell Proteomics. 2016 Jun;15(6):2021-32. doi: 10.1074/mcp.M115.057240. Epub 2016 Apr 7.
7
Phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 and its dynamics during nitrogen starvation.
Front Microbiol. 2015 Mar 31;6:248. doi: 10.3389/fmicb.2015.00248. eCollection 2015.
8
Toward the complete proteome of Synechocystis sp. PCC 6803.
Photosynth Res. 2015 Dec;126(2-3):203-19. doi: 10.1007/s11120-015-0140-y. Epub 2015 Apr 11.
9
Systematically ranking the tightness of membrane association for peripheral membrane proteins (PMPs).
Mol Cell Proteomics. 2015 Feb;14(2):340-53. doi: 10.1074/mcp.M114.044800. Epub 2014 Dec 13.
10
Proteomic approaches in research of cyanobacterial photosynthesis.
Photosynth Res. 2015 Oct;126(1):47-70. doi: 10.1007/s11120-014-0050-4. Epub 2014 Oct 31.

本文引用的文献

1
SIMPLE CONDITIONS FOR GROWTH OF UNICELLULAR BLUE-GREEN ALGAE ON PLATES(1, 2).
J Phycol. 1968 Mar;4(1):1-4. doi: 10.1111/j.1529-8817.1968.tb04667.x.
2
Mass spectrometry-based prokaryote gene annotation.
Proteomics. 2007 Nov;7(22):4053-65. doi: 10.1002/pmic.200700080.
5
Profiling signaling polarity in chemotactic cells.
Proc Natl Acad Sci U S A. 2007 May 15;104(20):8328-33. doi: 10.1073/pnas.0701103104. Epub 2007 May 9.
6
Proteomics of Synechocystis sp. PCC 6803. Identification of novel integral plasma membrane proteins.
FEBS J. 2007 Feb;274(3):791-804. doi: 10.1111/j.1742-4658.2006.05624.x.
8
Proteomic characterization of acid stress response in Synechocystis sp. PCC 6803.
Proteomics. 2006 Jun;6(12):3614-24. doi: 10.1002/pmic.200600033.
10
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验