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蓝藻光合作用研究中的蛋白质组学方法。

Proteomic approaches in research of cyanobacterial photosynthesis.

作者信息

Battchikova Natalia, Angeleri Martina, Aro Eva-Mari

机构信息

Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, 20014, Turku, Finland.

出版信息

Photosynth Res. 2015 Oct;126(1):47-70. doi: 10.1007/s11120-014-0050-4. Epub 2014 Oct 31.

DOI:10.1007/s11120-014-0050-4
PMID:25359503
Abstract

Oxygenic photosynthesis in cyanobacteria, algae, and plants is carried out by a fabulous pigment-protein machinery that is amazingly complicated in structure and function. Many different approaches have been undertaken to characterize the most important aspects of photosynthesis, and proteomics has become the essential component in this research. Here we describe various methods which have been used in proteomic research of cyanobacteria, and demonstrate how proteomics is implemented into on-going studies of photosynthesis in cyanobacterial cells.

摘要

蓝细菌、藻类和植物中的氧光合作用是由一种奇妙的色素蛋白机制完成的,其结构和功能极其复杂。人们采用了许多不同的方法来表征光合作用的最重要方面,蛋白质组学已成为这项研究的重要组成部分。在这里,我们描述了在蓝细菌蛋白质组学研究中使用的各种方法,并展示了蛋白质组学是如何应用于正在进行的蓝细菌细胞光合作用研究中的。

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Heterocyst-specific flavodiiron protein Flv3B enables oxic diazotrophic growth of the filamentous cyanobacterium Anabaena sp. PCC 7120.异形胞特异性黄素二铁蛋白Flv3B使丝状蓝细菌鱼腥藻Anabaena sp. PCC 7120能够在有氧条件下进行固氮生长。
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Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis.
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Mol Cell Proteomics. 2018 Sep;17(9):1650-1669. doi: 10.1074/mcp.RA118.000699. Epub 2018 May 30.
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The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II.利用先进质谱技术剖析光系统II的生命周期
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