Fisher A L, Morris M J, Gilbert J D
Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486.
Biol Mass Spectrom. 1991 Jul;20(7):408-14. doi: 10.1002/bms.1200200703.
MK-287 is a novel platelet-activating factor antagonist. A sensitive and specific gas chromatographic/mass spectrometric assay has been developed for the determination of the drug in serum and plasma. The assay utilizes an extraction with methyl-t-butyl ether and subsequent trimethylsilylation of the hydroxyl function. The gas chromatographic/mass spectrometric determinations are carried out with temperature-programmed capillary gas chromatography and ammonia negative chemical ionization mass spectrometry. The method has sufficient sensitivity, precision, accuracy and selectivity for the analysis of drug concentrations in clinical samples.
MK - 287是一种新型血小板激活因子拮抗剂。已开发出一种灵敏且特异的气相色谱/质谱分析法，用于测定血清和血浆中的该药物。该分析法采用甲基叔丁基醚萃取，随后对羟基功能进行三甲基硅烷化。气相色谱/质谱测定通过程序升温毛细管气相色谱法和氨负离子化学电离质谱法进行。该方法对于临床样品中药物浓度的分析具有足够的灵敏度、精密度、准确度和选择性。
