Lea Robert, Papalopulu Nancy, Amaya Enrique, Dorey Karel
The Healing Foundation Centre, Michael Smith Building, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
Dev Dyn. 2009 Jun;238(6):1467-79. doi: 10.1002/dvdy.21913.
Fibroblast growth factor (FGF) signalling plays a major role during early vertebrate development. It is involved in the specification of the mesoderm, control of morphogenetic movements, patterning of the anterior-posterior axis, and neural induction. In mammals, 22 FGF ligands have been identified, which can be grouped into seven subfamilies according to their sequence homology and function. We have cloned 17 fgf genes from Xenopus tropicalis and have analysed their temporal expression by RT-PCR and spatial expression by whole mount in situ hybridisation at key developmental stages. It reveals the diverse expression pattern of fgf genes during early embryonic development. Furthermore, our analysis shows the transient nature of expression of several fgfs in a number of embryonic tissues. This study constitutes the most comprehensive description of the temporal and spatial expression pattern of fgf ligands and receptors during vertebrate development to date. Developmental Dynamics 238:1467-1479, 2009. (c) 2009 Wiley-Liss, Inc.
成纤维细胞生长因子（FGF）信号传导在脊椎动物早期发育过程中起主要作用。它参与中胚层的特化、形态发生运动的控制、前后轴的模式形成以及神经诱导。在哺乳动物中，已鉴定出22种FGF配体，根据它们的序列同源性和功能可分为七个亚家族。我们从热带爪蟾中克隆了17个fgf基因，并通过RT-PCR分析了它们在关键发育阶段的时间表达，通过全胚胎原位杂交分析了它们的空间表达。这揭示了fgf基因在早期胚胎发育过程中的多样表达模式。此外，我们的分析表明，一些fgf在许多胚胎组织中的表达具有短暂性。这项研究构成了迄今为止对脊椎动物发育过程中fgf配体和受体的时间和空间表达模式最全面的描述。《发育动力学》238:1467 - 1479，2009年。（c）2009年威利 - 利斯公司。
