Chen Y-C, Tou J C, Jaczynski J
School of Nutrition, Chung Shan Medical Univ, Taichung, Taiwan.
J Food Sci. 2009 Mar;74(2):H31-9. doi: 10.1111/j.1750-3841.2008.01026.x.
Proteins and insolubles were recovered from whole Antarctic krill via novel isoelectric solubilization/precipitation using different pH treatments. The protein recovery yield was 45% to 50% (dry basis). The recovered proteins had higher (P < 0.05) content of essential amino acids (EAAs) and non-EAAs as well as higher (P < 0.05) ratio of total EAA/total AA than whole krill. The EAAs constituted almost 50% of total AAs. The least extreme pH treatments (pHs 3 and 12) yielded highest (P < 0.05) content of EAAs. The quality of recovered proteins was high based on EAAs meeting FAO/WHO/UNU recommendations for adults and infants. The basic pH yielded proteins with the lowest (P < 0.05) amount of minerals and the highest (P < 0.05) amount of Ca, P, and Mg in the insolubles when compared to the acidic treatments. However, both basic and acidic treatments effectively removed minerals from recovered proteins without the removal of the exoskeleton before processing. Therefore, besides high-quality proteins, the insolubles may provide a mineral supplement in the animal diet.
通过使用不同pH处理的新型等电溶解/沉淀方法,从整个南极磷虾中回收蛋白质和不溶性物质。蛋白质回收率为45%至50%(干基)。回收的蛋白质中必需氨基酸(EAA)和非必需氨基酸的含量更高(P<0.05),并且总EAA/总氨基酸的比例也高于整个磷虾(P<0.05)。必需氨基酸几乎占总氨基酸的50%。最温和的pH处理(pH值3和12)产生的必需氨基酸含量最高(P<0.05)。基于必需氨基酸符合粮农组织/世卫组织/联合国大学对成年人和婴儿的建议,回收蛋白质的质量很高。与酸性处理相比,碱性pH产生的蛋白质中矿物质含量最低(P<0.05),而不溶性物质中钙、磷和镁的含量最高(P<0.05)。然而,在加工前,碱性和酸性处理都能有效地从回收的蛋白质中去除矿物质,而无需去除外骨骼。因此,除了高质量的蛋白质外,不溶性物质还可以为动物饮食提供矿物质补充。