Rentsova I, Gurykh I
Vopr Med Khim. 1977 Jan-Feb(1):114-9.
Binding of iron with the molecule of prolyl hydroxylase was studied using 59Fe. The label was administered into one-day-old rats and within 36 hours the enzyme was isolated from skin. After the final step of purification (DEAE cellulose) 59Fe was not found in fractions of prolyl hydroxylase. The purity of the enzyme preparations was controlled by polyacrylamide gel disc electrophoresis. The data obtained suggest that Fe ions are not inherent in the enzyme molecule.
使用59Fe研究了铁与脯氨酰羟化酶分子的结合情况。将该标记物注射到1日龄大鼠体内，36小时内从皮肤中分离出该酶。在纯化的最后一步（DEAE纤维素）之后，在脯氨酰羟化酶的组分中未发现59Fe。通过聚丙烯酰胺凝胶圆盘电泳控制酶制剂的纯度。所得数据表明铁离子并非该酶分子所固有。
