Parr Christian, Jiang Wen G
Metastasis and Angiogenesis Research Group, Department of Surgery, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK.
Eur J Cancer. 2009 Jun;45(9):1673-83. doi: 10.1016/j.ejca.2009.02.019. Epub 2009 Mar 26.
Metastasis suppressor 1 (MTSS1) may play an important role in cancer metastasis. Firstly, this study assessed MTSS1 expression levels within breast cancer patients to reveal any clinical relevance. Secondly, we aimed to clarify the cellular function of MTSS1 in breast cancer cells. MTSS1 expression levels were assessed in a cohort of breast cancer specimens (normal n=33; cancer n=127), through quantitative PCR analysis and immuno-histochemical techniques. The influence of MTSS1 was further examined via biological overexpression and knockdown within breast cancer cell lines. We report that patients with tumours expressing reduced levels of MTSS1 had a poorer prognosis (p=0.042). High levels of MTSS1 correlated with an increased patient overall survival (p=0.0108) and disease-free survival (p=0.012). Furthermore, overexpression of MTSS1 significantly suppressed (p<0.01) the invasive, migratory, growth and adherence properties of a human breast cancer cell line. In contrast, knockdown of MTSS1 dramatically enhanced these properties. We conclude that MTSS1 is a prognostic indicator of disease-free survival in breast cancer patients and demonstrates the ability to play a role in governing the metastatic nature of breast cancer cells.
转移抑制因子1（MTSS1）可能在癌症转移中发挥重要作用。首先，本研究评估了乳腺癌患者体内MTSS1的表达水平，以揭示其临床相关性。其次，我们旨在阐明MTSS1在乳腺癌细胞中的细胞功能。通过定量PCR分析和免疫组织化学技术，对一组乳腺癌标本（正常n = 33；癌症n = 127）中的MTSS1表达水平进行了评估。通过在乳腺癌细胞系中进行生物学过表达和敲低，进一步研究了MTSS1的影响。我们报告说，MTSS1表达水平降低的肿瘤患者预后较差（p = 0.042）。MTSS1水平高与患者总生存期增加（p = 0.0108）和无病生存期增加（p = 0.012）相关。此外，MTSS1的过表达显著抑制（p <0.01）了人乳腺癌细胞系的侵袭、迁移、生长和黏附特性。相反，MTSS1的敲低显著增强了这些特性。我们得出结论，MTSS1是乳腺癌患者无病生存期的预后指标，并证明其在控制乳腺癌细胞转移特性方面具有作用。
