乳牙干细胞修复猪下颌骨缺损
Stem cells from deciduous tooth repair mandibular defect in swine.
作者信息
Zheng Y, Liu Y, Zhang C M, Zhang H Y, Li W H, Shi S, Le A D, Wang S L
机构信息
Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy & Tooth Regeneration, Capital Medical University School of Stomatology, Beijing, China.
出版信息
J Dent Res. 2009 Mar;88(3):249-54. doi: 10.1177/0022034509333804.
Stem cells from human exfoliated deciduous teeth have been identified as a new post-natal stem cell population with multipotential differentiation capabilities, including regeneration of mineralized tissues in vivo. To examine the efficacy of utilizing these stem cells in regenerating orofacial bone defects, we isolated stem cells from miniature pig deciduous teeth and engrafted the critical-size bone defects generated in swine mandible models. Our results indicated that stem cells from miniature pig deciduous teeth, an autologous and easily accessible stem cell source, were able to engraft and regenerate bone to repair critical-size mandibular defects at 6 months post-surgical reconstruction. This pre-clinical study in a large-animal model, specifically swine, allows for testing of a stem cells/scaffold construct in the restoration of orofacial skeletal defects and provides rapid translation of stem-cell-based therapy in orofacial reconstruction in human clinical trials.
人脱落乳牙干细胞已被鉴定为一种新的出生后具有多能分化能力的干细胞群体，包括在体内矿化组织的再生。为了研究利用这些干细胞再生口腔颌面部骨缺损的效果，我们从小型猪乳牙中分离出干细胞，并将其植入猪下颌骨模型中产生的临界尺寸骨缺损处。我们的结果表明，小型猪乳牙干细胞是一种自体且易于获取的干细胞来源，在手术重建后6个月能够植入并再生骨组织以修复临界尺寸的下颌骨缺损。这项在大型动物模型（特别是猪）中进行的临床前研究，允许测试干细胞/支架构建物在修复口腔颌面部骨骼缺损中的应用，并为人类临床试验中基于干细胞的口腔颌面重建治疗提供快速转化。
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