低剂量和极低剂量的简单酚类物质对植物过氧化物酶活性的影响。
Effect of low and very low doses of simple phenolics on plant peroxidase activity.
作者信息
Malarczyk Elzbieta, Kochmańska-Rdest Janina, Paździoch-Czochra Marzanna
机构信息
Department of Biochemistry, M. Curie-Skłodowska University, Lublin, Poland.
出版信息
Nonlinearity Biol Toxicol Med. 2004 Apr;2(2):129-41. doi: 10.1080/15401420490464466.
Changes in the activity of horseradish peroxidase resulting from an addition of ethanol water dilutions of 19 phenolic compounds were observed. For each compound, the enzyme activity was plotted against the degree of dilution expressed as n = -log(100) (mol/L) in the range 0 </= n >/= 20. All the curves showed sinusoidal activity, more or less regular, with two to four peaks on average. Each analyzed compound had a characteristic sinusoidal shape, which was constant for samples of peroxidase from various commercial firms. This was clearly visible after function fitting to experimental results based on the Marquadt-Levenberg algorithm using the least-squares method. Among the 19 phenolics, the highest amplitudes were observed for phenol and iso- and vanillate acids and aldehydes. The specific character of each of the analyzed curves offers a possibility of choosing proper dilutions of phenolic compound for activating or inhibiting of peroxidase activity.
观察了添加19种酚类化合物的乙醇 - 水稀释液后辣根过氧化物酶活性的变化。对于每种化合物，以酶活性对稀释度作图，稀释度用n = -log(100)（mol/L）表示，范围为0 ≤ n ≤ 20。所有曲线均呈现出或多或少规则的正弦活性，平均有两到四个峰值。每种分析的化合物都有其特征性的正弦形状，对于来自不同商业公司的过氧化物酶样品来说是恒定的。基于最小二乘法使用Marquadt - Levenberg算法对实验结果进行函数拟合后，这一点清晰可见。在这19种酚类物质中，苯酚、异香草酸和香草酸以及醛类的振幅最高。每条分析曲线的特异性为选择合适的酚类化合物稀释液以激活或抑制过氧化物酶活性提供了可能。
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