Li Xiaofeng, Liu Yinglong, Wang Qiang, Zhu Yaobin, Lv Xiaodong, Liu Jinping
Peking Union Medical College and Chinese Academy of Medical Sciences, Pediatric Cardiac Surgery Center, Cardiovascular Institute and Fuwai Hospital, Beijing, PR China.
Acta Vet Scand. 2009 Mar 30;51(1):17. doi: 10.1186/1751-0147-51-17.
Children are susceptible to pulmonary injury, and acute lung injury (ALI) often results in a high mortality and financial cost in pediatric patients. Evidence has showed that oleic acid (OA) plays an important role in ALI. Therefore, it has special significance to study ALI in pediatric patients by using OA-induced animal models. Unfortunately, the animal model hs a high mortality due to hemodynamic instability. The aim of this study was to establish a novel hemodynamically stable OA-induced ALI model in piglets with two hits.
18 Chinese mini-piglets were randomized into three groups: group C (received saline-ethanol solution), group T (received OA-ethanol solution in routine administration manner) and group H (received OA-ethanol solution in two-hit manner). Hemodynamic and pulmonary function data were measured. Histopathological assessments were performed.
Two piglets in group T died of radical decline of systemic blood pressure. Group T showed more drastic hemodynamic changes than group H especially during the period of 5 to 30 minutes after OA administration. Both Group T and group H all produced severe lung injury, while group C had no significant pathologic changes. OA-induced hypotension might be caused by pulmonary hypertension rather than comprised left ventricular function.
OA leads to severe pulmonary hypertension which results in hemodynamic fluctuation in OA-induced ALI model. It is the first report on hemodynamic stable ALI animal model in piglets using two-hit method. The two-hit ALI animal model fulfils the ALI criteria and has the following characteristics: hemodynamic stability, stable damage to gas exchange and comparability with pediatric patients in body weight and corresponding age. The two-hit ALI animal model can be used to study the basic mechanism and the therapeutic strategies for pediatric ALI.
儿童易发生肺损伤，急性肺损伤（ALI）在儿科患者中常导致高死亡率和高昂的经济成本。有证据表明，油酸（OA）在ALI中起重要作用。因此，利用OA诱导的动物模型研究儿科患者的ALI具有特殊意义。不幸的是，该动物模型因血流动力学不稳定而死亡率高。本研究的目的是建立一种新型的、血流动力学稳定的、采用两次打击法的OA诱导的仔猪ALI模型。
将18只中国小型仔猪随机分为三组：C组（接受生理盐水 - 乙醇溶液）、T组（以常规给药方式接受OA - 乙醇溶液）和H组（以两次打击方式接受OA - 乙醇溶液）。测量血流动力学和肺功能数据。进行组织病理学评估。
T组有2只仔猪死于全身血压急剧下降。T组比H组表现出更剧烈的血流动力学变化，尤其是在OA给药后5至30分钟期间。T组和H组均产生了严重的肺损伤，而C组无明显病理变化。OA诱导的低血压可能是由肺动脉高压引起的，而非左心室功能受损。
OA导致严重的肺动脉高压，从而在OA诱导的ALI模型中引起血流动力学波动。这是首次关于采用两次打击法建立的仔猪血流动力学稳定的ALI动物模型的报道。两次打击ALI动物模型符合ALI标准，具有以下特点：血流动力学稳定、气体交换损伤稳定、体重和相应年龄与儿科患者具有可比性。两次打击ALI动物模型可用于研究儿科ALI的基本机制和治疗策略。
