Clark C L, Price B A, Malcolm P, Lear P A, Wood R F
Professional Surgical Unit, St. Bartholomew's Hospital, West Smithfield, London, UK.
Br J Surg. 1991 Sep;78(9):1077-9. doi: 10.1002/bjs.1800780915.
Quantities of organized lymphoid tissue in small bowel allografts may cause graft versus host disease (GVHD) following transplantation. This study examines the effect of graft mesenteric lymphadenectomy on development of GVHD following small bowel transplantation in rats. GVH reactivity was assessed by measuring the degree of graft cell emigration to the host. In the PVG to DA strain combination, graft mesenteric lymphadenectomy led to a significant reduction in graft cell colonization of host lymphoid tissues from 40-50 per cent to 25-35 per cent. Transplantation from PVG to (PVG x DA)F1 hybrids caused fatal GVHD within 21 days whereas when DA donors were used survival was over 30 days. When mesenteric lymphadenectomy was performed on PVG donors, host survival increased by only 3-4 days. Mesenteric lymphadenectomy in DA donors led to long-term recipient survival with no GVHD. Intensity of GVHD following rat small bowel transplantation is a strain-dependent phenomenon and graft mesenteric lymphadenectomy does not always prevent GVHD. The mucosa may have an important immunological role.
小肠同种异体移植中大量有组织的淋巴组织可能在移植后引发移植物抗宿主病（GVHD）。本研究探讨了移植肠系膜淋巴结切除对大鼠小肠移植后GVHD发生的影响。通过测量移植物细胞向宿主迁移的程度来评估移植物抗宿主反应性。在PVG到DA品系组合中，移植肠系膜淋巴结切除导致宿主淋巴组织中移植物细胞定植率从40%-50%显著降低至25%-35%。从PVG移植到（PVG×DA）F1杂种在21天内导致致命的GVHD，而使用DA供体时，生存期超过30天。当对PVG供体进行肠系膜淋巴结切除时，宿主生存期仅延长3-4天。对DA供体进行肠系膜淋巴结切除可使受体长期存活且无GVHD。大鼠小肠移植后GVHD的强度是一种品系依赖性现象，移植肠系膜淋巴结切除并不总能预防GVHD。黏膜可能具有重要的免疫作用。
