Jensen A B, James R R, Eilenberg J
Department of Ecology and Agriculture, University of Copenhagen, Frederiksberg C, Denmark.
J Invertebr Pathol. 2009 Jun;101(2):157-60. doi: 10.1016/j.jip.2009.03.004. Epub 2009 Mar 28.
Survival rates of Ascosphaera aggregata and Ascosphaera apis over the course of a year were tested using different storage treatments. For spores, the storage methods tested were freeze-drying and ultra-low temperatures, and for hyphae, freeze-drying, agar slants, and two methods of ultra-low temperatures. Spores of A. aggregata and A. apis stored well at -80 degrees C and after freeze-drying. A. aggregata hyphae did not store well under any of the methods tested while A. apis hyphae survived well using cryopreservation. Spores produced from cryopreserved A. apis hyphae were infective. Long-term storage of these two important fungal bee diseases is thus possible.
使用不同的储存处理方法测试了一年中聚集球囊菌和蜜蜂球囊菌的存活率。对于孢子，测试的储存方法是冷冻干燥和超低温，对于菌丝体，测试的储存方法是冷冻干燥、琼脂斜面和两种超低温方法。聚集球囊菌和蜜蜂球囊菌的孢子在-80摄氏度和冷冻干燥后储存良好。聚集球囊菌的菌丝体在任何测试方法下都储存不佳，而蜜蜂球囊菌的菌丝体通过冷冻保存存活良好。由冷冻保存的蜜蜂球囊菌菌丝体产生的孢子具有传染性。因此，这两种重要的蜜蜂真菌病害能够长期保存。
