一种用于测定头孢曲松对伤寒沙门氏菌和副伤寒沙门氏菌最低抑菌浓度的简单肉汤-纸片法。
A simple broth-disk method to determine the minimum inhibitory concentration of ceftriaxone on Salmonella enterica serovar typhi and paratyphi.
作者信息
Bhat K Gopalkrishna, Tripathy Amruta, Rajagopal Rajasree, Ramachandran Sreeja
机构信息
Department of Microbiology, Kasturba Medical College, Mangalore-575 001, India.
出版信息
Indian J Pathol Microbiol. 2009 Apr-Jun;52(2):189-90. doi: 10.4103/0377-4929.48913.
BACKGROUND AND PURPOSE
Resistance to fluoroquinolones and cephalosporins is a major problem with Salmonella enterica serovar Typhi and Paratyphi. An accurate determination of antibiotic susceptibility requires tests for minimum inhibitory concentration (MIC) of antibiotics. We describe a simple broth-disk method to determine the MIC of ceftriaxone on S. typhi and S. paratyphi.
MATERIALS AND METHODS
Sixteen strains of S. typhi and two strains each of S. paratyphi A and S. paratyphi B were used in the study. The MIC of ceftriaxone was determined using the simple broth-disk method and the conventional broth macrodilution method and the results were compared.
RESULTS
All salmonella strains were susceptible to ceftriaxone. The results of the broth-disk and the conventional broth macrodilution method were similar.
CONCLUSION
The broth-disk method is a simple, reliable and cost-effective method to determine the MIC of ceftriaxone on S. typhi and S. paratyphi A.
背景与目的
对氟喹诺酮类和头孢菌素类药物耐药是伤寒沙门菌和副伤寒沙门菌的一个主要问题。准确测定抗生素敏感性需要进行抗生素最低抑菌浓度（MIC）检测。我们描述了一种简单的肉汤纸片法来测定头孢曲松对伤寒沙门菌和副伤寒沙门菌的MIC。
材料与方法
本研究使用了16株伤寒沙门菌以及各2株甲型副伤寒沙门菌和乙型副伤寒沙门菌。采用简单肉汤纸片法和传统肉汤稀释法测定头孢曲松的MIC，并比较结果。
结果
所有沙门菌菌株对头孢曲松敏感。肉汤纸片法和传统肉汤稀释法的结果相似。
结论
肉汤纸片法是一种简单、可靠且经济高效的方法，可用于测定头孢曲松对伤寒沙门菌和甲型副伤寒沙门菌的MIC。
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