Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India.
Appl Biochem Biotechnol. 2010 Mar;160(5):1267-76. doi: 10.1007/s12010-009-8593-0. Epub 2009 Mar 31.
Phytase of the thermophilic mold Sporotrichum thermophile Apinis hydrolyzed and liberated inorganic phosphate from Ca(+2), Mg(+2), and Co(+2) phytates more efficiently than those of Al(3+), Fe(2+), Fe(3+), and Zn(2+). The hydrolysis rate was higher at 60 degrees C as compared to 26 degrees Celsius. Among all the organic acids tested, citrate was more effective in enhancing solubilization of insoluble phytate salts by phytase than others. The dry weight and inorganic phosphate contents of the wheat plants were high when supplemented with phytase or fungal spores. The plants provided with 5 mg phytate per plant exhibited enhanced growth and inorganic phosphate. With increase in the dosage of phytase, there was increase in growth and inorganic phosphate of plants, the highest being at 20 U per plant. The compost made employing the combined native microflora of the wheat straw and S. thermophile promoted growth of the plants. The plant-growth-promoting effect was also higher with the compost made using S. thermophile than that from only the native microflora.
嗜热真菌嗜热曲霉的植酸酶能够更有效地水解 Ca(+2)、Mg(+2) 和 Co(+2)植酸盐并释放无机磷，而对 Al(3+)、Fe(2+)、Fe(3+)和 Zn(2+)植酸盐的水解效率较低。与 26°C 相比，60°C 时的水解速率更高。在所有测试的有机酸中，柠檬酸比其他有机酸更有效地促进植酸酶对不溶性植酸盐的溶解。当小麦植株补充植酸酶或真菌孢子时，其干重和无机磷含量较高。每株植物补充 5mg 植酸盐可促进植物生长和无机磷的吸收。随着植酸酶剂量的增加，植物的生长和无机磷含量也随之增加，最高可达每株 20U。采用小麦秸秆和嗜热曲霉的混合土著微生物制成的堆肥促进了植物的生长。使用嗜热曲霉制成的堆肥比仅使用土著微生物制成的堆肥对植物的促生长效果更高。
