Singh Bijender, Satyanarayana T
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110 021, India.
Appl Biochem Biotechnol. 2006 Jun;133(3):239-50. doi: 10.1385/abab:133:3:239.
The phytase production by Sporotrichum thermophile TLR50 was recorded on all the commonly used animal feed ingredients tested to varying degrees in solid-state fermentation. Enzyme production increased to 180 U/g of dry moldy residue (DMR) in sesame oil cake at 120 h and 45 degrees C at the initial substrate-to-moisture ratio of 1:2.5 and aw of 0.95. Supplementation of sesame oil cake with glucose and ammonium sulfate further enhanced phytase titer (282 U/g of DMR). An overall 76% enhancement in phytase production was achieved owing to optimization. The mold secreted acid phosphatase, amylase, xylanase, and lipase along with phytase. By the action of phytase, inorganic phosphate was liberated efficiently, leading to dephytinization of sesame oil cake.
嗜热枝孢霉TLR50在固态发酵中对所有测试的常用动物饲料成分都不同程度地产生植酸酶。在初始底物与水分比为1:2.5、水分活度为0.95的条件下，于45℃培养120 h时，芝麻油饼中的酶产量增加到180 U/g干霉菌残渣（DMR）。用葡萄糖和硫酸铵补充芝麻油饼可进一步提高植酸酶效价（282 U/g DMR）。通过优化，植酸酶产量总体提高了76%。该霉菌除了分泌植酸酶外，还分泌酸性磷酸酶、淀粉酶、木聚糖酶和脂肪酶。通过植酸酶的作用，无机磷酸盐被有效释放，从而实现了芝麻油饼的脱植酸。
