Department of Chemistry, China Agricultural University, 2 Yuan-ming-yuan xi Road, Haidian District, Beijing 100193, China.
J Fluoresc. 2009 Sep;19(5):783-91. doi: 10.1007/s10895-009-0475-x. Epub 2009 Mar 31.
The interaction of a N-methylated diaminotriphenylmethane dye, malachite green, with lysozyme was investigated by fluorescence spectroscopic techniques under physiological conditions. The binding parameters have been evaluated by fluorescence quenching methods. The results revealed that malachite green caused the fluorescence quenching of lysozyme through a static quenching procedure. The thermodynamic parameters like DeltaH and DeltaS were calculated to be -15.33 kJ mol(-1) and 19.47 J mol(-1) K(-1) according to van't Hoff equation, respectively, which proves main interaction between malachite green and lysozyme is hydrophobic forces and hydrogen bond contact. The distance r between donor (lysozyme) and acceptor (malachite green) was obtained to be 3.82 nm according to Frster's theory. The results of synchronous fluorescence, UV/vis and three-dimensional fluorescence spectra showed that binding of malachite green with lysozyme can induce conformational changes in lysozyme. In addition, the effects of common ions on the constants of lysozyme-malachite green complex were also discussed.
在生理条件下，利用荧光光谱技术研究了 N-甲基化二氨基三苯甲烷染料孔雀石绿与溶菌酶的相互作用。通过荧光猝灭法评估了结合参数。结果表明，孔雀石绿通过静态猝灭过程引起溶菌酶的荧光猝灭。根据范特霍夫方程，热力学参数如ΔH 和 ΔS 的计算值分别为-15.33 kJ/mol 和 19.47 J/mol/K，这证明了孔雀石绿和溶菌酶之间的主要相互作用是疏水作用力和氢键接触。根据 Frster 理论，获得供体（溶菌酶）和受体（孔雀石绿）之间的距离 r 为 3.82 nm。同步荧光、UV/vis 和三维荧光光谱的结果表明，孔雀石绿与溶菌酶的结合可以诱导溶菌酶构象发生变化。此外，还讨论了常见离子对溶菌酶-孔雀石绿配合物常数的影响。
