State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, People's Republic of China.
Environ Monit Assess. 2010 Apr;163(1-4):607-17. doi: 10.1007/s10661-009-0863-3. Epub 2009 Mar 31.
The distributions of culturable bacteria and functional bacteria associated with nitrogen (N) or phosphorus (P) in the backwater areas of the Three Gorges Reservoir (TGR) were investigated. Results from seven locations in the TGR indicated that the abundance of total bacteria was high, with 8.12 x 10(6), 2.70 x 10(7), and 6.73 x 10(10) colony-forming units per milliliter or per gram dry weight in surface water, bottom water, and sediments, respectively. Aquatic environments with higher nutrient loadings possessed higher bacteria densities and lower bacteria community diversities. Eight kinds of functional bacteria ratios, including surface water to bottom water and ratios of water to sediments, were calculated, in which four kinds of functional bacteria, namely, nitrogen-fixing bacteria, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and organophosphate-solubilizing bacteria, displayed obvious differences in different locations. Based on the functional bacteria ratios of water to sediments, it was found that the obtained result of location grouping from cluster analysis was similar to that based on the community-level physiological profiles studies. The above results showed that the ratios of functional bacteria could distinguish the aquatic environments with different trophic conditions in the TGR. This demonstrated that the distribution ratios of functional bacteria in aquatic environments could work as potential bioindicators to reflect the trophic condition of the water.
调查了三峡水库（TGR）回水区域可培养细菌和与氮（N）或磷（P）相关的功能细菌的分布。TGR 七个地点的结果表明，总细菌丰度较高，分别为每毫升或每克干重地表水、底水和沉积物中的 8.12x10(6)、2.70x10(7)和 6.73x10(10) 菌落形成单位。具有较高养分负荷的水生环境具有更高的细菌密度和更低的细菌群落多样性。计算了 8 种功能细菌的比值，包括地表水与底水的比值以及水与沉积物的比值，其中 4 种功能细菌，即固氮菌、氨氧化菌、亚硝酸盐氧化菌和有机磷溶解菌，在不同地点表现出明显的差异。基于水与沉积物的功能细菌比值，发现聚类分析得出的位置分组结果与基于群落水平生理图谱研究的结果相似。上述结果表明，功能细菌的比值可以区分 TGR 中具有不同营养条件的水生态环境。这表明水生环境中功能细菌的分布比值可以作为潜在的生物指标来反映水的营养状况。
