Gui Qin, Wang Jialu, Xu Yanhao, Wang Jianbo
Mol Biol (Mosk). 2009 Jan-Feb;43(1):3-9.
Polyploidization plays an important role in plant evolution, and it may trigger changes in genome structure and gene expression. The purpose of this study was to detect the extent of gene expression alterations in natural Brassica allotetraploids. Sequence-related amplified polymorphism (SRAP)-cDNA display was carried out on six species (13 accessions), three allotetraploids and their three ancestral diploids. Total of 1496 transcripts were screened, finding that 92 (6.1%) transcripts were subjected to silencing in allopolyploids, while 19 (1.3%) transcripts were activated. The results indicated that a significant fraction of duplicated genes have shown expression alterations in the natural allotetraploids compared with their diploid parents.
多倍体化在植物进化中起着重要作用，它可能引发基因组结构和基因表达的变化。本研究的目的是检测天然芸苔属异源四倍体中基因表达改变的程度。对六个物种（13个材料）、三个异源四倍体及其三个祖先二倍体进行了序列相关扩增多态性（SRAP）-cDNA展示。共筛选了1496个转录本，发现92个（6.1%）转录本在异源多倍体中发生沉默，而19个（1.3%）转录本被激活。结果表明，与其二倍体亲本相比，相当一部分重复基因在天然异源四倍体中表现出表达改变。
