Zhang Y, Chen B, Li Y P, Lou G Y, Chen M, Zhou D J
Mol Biol (Mosk). 2009 Jan-Feb;43(1):77-84.
PNRC (Proline-rich Nuclear Receptor Coactivator) is a novel coactivator for multiple nuclear receptors. PNRC was previously identified using bovine SF-1 (steroidogenic factor 1) as the bait in a yeast two-hybrid screening of a human mammary gland cDNA expression library. To understand the molecular mechanisms that regulate the expression of human PNRC gene, in this study, functional analysis of the 5' flanking region of the human PNRC gene revealed that the -123/+27 region is the minimal promoter of the human PNRC gene. Gel shift and ChIP analyses demonstrated the specific binding of RFX1 (Regulatory Factor X) protein to the human PNRC promoter region. In co-transfection experiments RFX1 was shown to repress promoter activity of PNRC gene in a dose-dependent manner. These results indicate that r RFX1 specifically bind to promoter region and negatively regulate the transcription of the human PNRC gene.
富含脯氨酸的核受体共激活因子（PNRC）是一种新型的多种核受体共激活因子。PNRC先前是在以牛类固醇生成因子1（SF-1）为诱饵对人乳腺cDNA表达文库进行酵母双杂交筛选时被鉴定出来的。为了了解调控人PNRC基因表达的分子机制，在本研究中，对人PNRC基因5'侧翼区域的功能分析表明，-123 / +27区域是人PNRC基因的最小启动子。凝胶迁移和染色质免疫沉淀分析证明了调控因子X1（RFX1）蛋白与人PNRC启动子区域的特异性结合。在共转染实验中，RFX1以剂量依赖的方式抑制PNRC基因的启动子活性。这些结果表明，RFX1特异性结合启动子区域并负向调节人PNRC基因的转录。
