Wu H T, Yan X M, Hu Y L, Diao Z Y, Wu J, Zhang S Q
Mol Biol (Mosk). 2009 Jan-Feb;43(1):85-90.
B cell-activating factor of the TNF family (BAFF) is critical for B cell maturation and survival. Here, we constructed a stable CHO cell line, in which the expression level of soluble form of BAFF (sBAFF) was raised from 0.13 microg/ml to 0.55 microg/ml. Purified recombinant sBAFF from these CHO cells not only bound to its receptors but also co-stimulated the proliferation of human peripheral blood B lymphocyte in vitro. These results provided us with a useful basis for further studies about sBAFF-related research.
肿瘤坏死因子家族的B细胞激活因子（BAFF）对B细胞的成熟和存活至关重要。在此，我们构建了一个稳定的CHO细胞系，其中可溶性BAFF（sBAFF）的表达水平从0.13微克/毫升提高到了0.55微克/毫升。从这些CHO细胞中纯化得到的重组sBAFF不仅能与其受体结合，还能在体外共刺激人外周血B淋巴细胞的增殖。这些结果为我们进一步开展与sBAFF相关的研究提供了有用的基础。
