[共固定化细胞因子诱导HeLa细胞凋亡中Bax、Bcl-2和P53的表达]

[Expression of Bax, Bcl-2 and P53 in the HeLa apoptosis induced by co-immobilized cytokines].

作者信息

Guan Yanqing, Qiu Lili, Wang Wenwen

机构信息

College of Life Science, South China Normal University, Guangzhou 510631, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Feb;26(1):138-43.

PMID:19334572

Abstract

The aim of this study was to evaluate the changes in the expression of Bax, Bcl-2 and P53 when HeLa cells were induced by free cytokines or co-immobilized cytokines. The cells were induced for 24 hrs,72 hrs, 120 hrs and 168 hrs. Then, the expression of Bax, Bcl-2 and P53 was observed by immunohistochemistry. The average optic density of reaction products was tested by image analysis. Lastly, data were analyzed statistically. After the HeLa cells were induced for 120 hrs, the average optic denisity of Bcl-2 was much decreased. However, the average optic Bax was much increased. The average optic density of P53 also increased with the increase of time. The results suggest that HeLa apoptosis was induced by tumor necrosis factor-a and interferon -gamma, and the increasing expression of P53 may induce the expression of Bax and prevent the expression of Bcl-2, via the mitochondrial induction of cell apoptosis.

摘要

本研究的目的是评估游离细胞因子或共固定化细胞因子诱导人宫颈癌HeLa细胞时，Bax、Bcl-2和P53表达的变化。将细胞诱导24小时、72小时、120小时和168小时。然后，通过免疫组织化学观察Bax、Bcl-2和P53的表达。通过图像分析检测反应产物的平均光密度。最后，对数据进行统计学分析。HeLa细胞诱导120小时后，Bcl-2的平均光密度显著降低。然而，Bax的平均光密度显著增加。P53的平均光密度也随时间增加而增加。结果表明，肿瘤坏死因子-α和干扰素-γ可诱导HeLa细胞凋亡，P53表达增加可能通过线粒体诱导细胞凋亡，诱导Bax表达并抑制Bcl-2表达。