[CD4-CD8-T细胞在3型鼠肝炎病毒诱导的慢性病毒性肝炎中的作用]

[Role of CD4-CD8- T cells in the murine hepatitis virus type 3 induced chronic viral hepatitis].

作者信息

Wang Xiao-Jing, Yan Wei-Ming, Zhang Jiang-Guo, Wang Hong-Wu, Zou Yong, Luo Xiao-Ping, Ning Qin

机构信息

Institute of Infectious Disease, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2009 Mar;17(3):180-3.

PMID:19335979

Abstract

OBJECTIVE

To investigate role of CD4-CD8- T cells in murine hepatitis virus type 3 (MHV-3) induced chronic viral hepatitis in C3H/Hej mice and to identify their surface markers.

METHODS

Thirty C3H/Hej mice received 10 Pfu MHV-3 intraperitoneally, the CD4-CD8- T cells were isolated using magnetic bead sorting on 0, 4, 15, 30, 40 days post MHV-3 infection. The cytotoxic effects of CD4-CD8- T cells on normal and infected hepatocytes, CD8+ T cells and unrelated-virus (murine cytomegalovirus, MCMV) infected CD8+ T cells were examined by non-radioactive cytotoxicity assay. The surface markers of CD4-CD8- T cells were determined by flow cytometry.

RESULTS

MHV-3 infected CD4-CD8- T cells showed significant cytotoxic effect on CD8+ T cells, but not on infected hepatocytes or MCMV infected CD8+ T cells. The analysis of cell surface markers demonstrated that the CD4-CD8- T cells are a completely new T cell subset.

CONCLUSIONS

CD4-CD8- T cells have significant cytotoxic effect on virus specific CD8+ T cells in MHV-3 infected C3H/Hej mice, which suggests that CD4-CD8- T cells have immune modulatory functions in the development of chronic viral hepatitis. The phenotype of these CD4-CD8- T cells detected by flow cytometry is TCR alpha beta +CD3+CD4- CD8- CD25- CD28- CD30- CD44+.

摘要

目的

研究CD4-CD8-T细胞在3型鼠肝炎病毒（MHV-3）诱导的C3H/Hej小鼠慢性病毒性肝炎中的作用，并鉴定其表面标志物。

方法

30只C3H/Hej小鼠腹腔注射10个噬斑形成单位（Pfu）的MHV-3，在MHV-3感染后的0、4、15、30、40天，使用磁珠分选法分离CD4-CD8-T细胞。通过非放射性细胞毒性试验检测CD4-CD8-T细胞对正常和感染的肝细胞、CD8+T细胞以及无关病毒（鼠巨细胞病毒，MCMV）感染的CD8+T细胞的细胞毒性作用。通过流式细胞术测定CD4-CD8-T细胞的表面标志物。

结果

MHV-3感染的CD4-CD8-T细胞对CD8+T细胞显示出显著的细胞毒性作用，但对感染的肝细胞或MCMV感染的CD8+T细胞无此作用。细胞表面标志物分析表明，CD4-CD8-T细胞是一个全新的T细胞亚群。

结论

在MHV-3感染的C3H/Hej小鼠中，CD4-CD8-T细胞对病毒特异性CD8+T细胞具有显著的细胞毒性作用，这表明CD4-CD8-T细胞在慢性病毒性肝炎的发展中具有免疫调节功能。通过流式细胞术检测到的这些CD4-CD8-T细胞的表型为TCRαβ+CD3+CD4-CD8-CD25-CD28-CD30-CD44+。