Sun Boqian, Chu Dake, Li Wei, Chu Xiaodan, Li Yunming, Wei Dun, Li Haiping
Medical Department of Neurology, Affiliated Hospital of Beihua University, 132011, Jilin, People's Republic of China.
J Neurooncol. 2009 Sep;94(2):213-9. doi: 10.1007/s11060-009-9859-7. Epub 2009 Apr 1.
The aim of the study was to examine the expression of NDRG1 gene in glioma samples with different WHO grades and its association with survival. About 168 glioma specimens and 21 normal control tissues were collected. Immunochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of NDRG1 and Myc. Kaplan-Meier method and Cox's proportional hazards model were used in survival analysis. Immunohistochemistry showed that Ndrg1 expression was reduced in glioma. NDRG1 mRNA and protein levels were lower in glioma compared to control on real-time PCR and Western blot analysis (P < 0.001). Its expression levels increase from grade IV to grade I glioma on real-time PCR, immunohistochemistry analysis (P < 0.001) and Western blot. On the contrary, the expression of Myc by real-time PCR and Western blot showed the opposite trend of NDRG1. The survival rate of Ndrg1-negative patients was lower than that of Ndrg1-positive patients. We confirmed that the loss of NDRG1 expression was a significant and independent prognostic indicator in glioma by multivariate analysis. NDRG1 may play an inhibitory role during the development of glioma and may be a potential prognosis predictor of glioma.
本研究旨在检测不同世界卫生组织（WHO）分级的胶质瘤样本中NDRG1基因的表达及其与生存的关系。收集了约168例胶质瘤标本和21例正常对照组织。采用免疫组织化学检测、定量实时聚合酶链反应（PCR）和蛋白质印迹分析来研究NDRG1和Myc的表达。生存分析采用Kaplan-Meier法和Cox比例风险模型。免疫组织化学显示，胶质瘤中Ndrg1表达降低。实时PCR和蛋白质印迹分析显示，与对照相比，胶质瘤中NDRG1 mRNA和蛋白水平较低（P < 0.001）。实时PCR、免疫组织化学分析（P < 0.001）和蛋白质印迹结果显示，其表达水平从IV级到I级胶质瘤逐渐升高。相反，实时PCR和蛋白质印迹检测的Myc表达与NDRG1呈相反趋势。Ndrg1阴性患者的生存率低于Ndrg1阳性患者。多因素分析证实，NDRG1表达缺失是胶质瘤重要的独立预后指标。NDRG1可能在胶质瘤发生发展过程中起抑制作用，可能是胶质瘤潜在的预后预测指标。
