Yuan Ye, Hwang Ji-Young, Krishnamoorthy Mowleswaran, Ye Kuntao, Zhang Yuan, Ning Jin, Wang Roy C, Deen M Jamal, Fang Qiyin
Department of Engineering Physics, School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada.
Opt Lett. 2009 Apr 1;34(7):1132-4. doi: 10.1364/ol.34.001132.
Time-resolved fluorescence spectroscopy has been studied to perform minimally invasive optical biopsy in clinical diagnostics. A critical barrier preventing current time-resolved techniques from clinical studies is their impractically long data acquisition time. We have developed an acousto-optic-tunable-filter (AOTF)-based time-resolved fluorescence spectrometer, which is capable of near real-time data acquisition. Both first-order diffraction beams are collected in this AOTF spectrometer, which results in significantly improved overall throughput. Using standard fluorescence dyes, we have demonstrated that this spectrometer can acquire 200 nm time-resolved spectra within 4 s, while its throughput is comparable to a grating-based spectrometer.
时间分辨荧光光谱技术已被用于临床诊断中的微创光学活检研究。阻碍当前时间分辨技术应用于临床研究的一个关键障碍是其数据采集时间过长，不切实际。我们开发了一种基于声光可调谐滤波器（AOTF）的时间分辨荧光光谱仪，它能够进行近实时数据采集。在这种AOTF光谱仪中，一阶衍射光束都被收集起来，这显著提高了整体通量。使用标准荧光染料，我们已经证明，这种光谱仪能够在4秒内采集200纳米的时间分辨光谱，同时其通量与基于光栅的光谱仪相当。
