Hammel I, Arizono N, Galli S J
Department of Pathology, Sackler School of Medicine, Tel Aviv University, Israel.
Cell Tissue Res. 1991 Aug;265(2):329-34. doi: 10.1007/BF00398080.
The cytoplasmic granules of mast cells have a periodic multimodal size distribution in which the volumes of individual granules are integral multiples of the intermodal distance, a volume defined as the "unit granule" or v1. In this study, we used two 3-month-old male rats to analyze two classical mast cell subpopulations, dermal "connective tissue-type mast cells" and jejunal lamina propria "mucosal mast cells", for the morphometric characteristics of their cytoplasmic granules. Both v1 and the mean volume of individual cytoplasmic granules were much smaller in dermal than in jejunal mast cells (ratios of 1:5.5 and 1:4.2, respectively), but dermal mast cells contained 150% more granules per cell than did jejunal mast cells. The two types of mast cells did not differ significantly in total cell volume, nucleus volume, aggregate volume of cytoplasmic granules per cell or numbers of unit granules comprising a granule of mean volume. These findings add unit granule volume to the list of phenotypic characteristics which express significant variation in anatomically distinct populations of mast cells.
肥大细胞的胞质颗粒具有周期性多峰大小分布，其中单个颗粒的体积是峰间距离的整数倍，该体积定义为“单位颗粒”或v1。在本研究中，我们使用两只3个月大的雄性大鼠来分析两种经典的肥大细胞亚群，即皮肤“结缔组织型肥大细胞”和空肠固有层“黏膜肥大细胞”，以研究其胞质颗粒的形态计量学特征。皮肤肥大细胞的v1和单个胞质颗粒的平均体积均比空肠肥大细胞小得多（比例分别为1:5.5和1:4.2），但皮肤肥大细胞每个细胞所含的颗粒比空肠肥大细胞多50%。这两种类型的肥大细胞在总细胞体积、细胞核体积、每个细胞的胞质颗粒聚集体积或构成平均体积颗粒的单位颗粒数量方面没有显著差异。这些发现将单位颗粒体积添加到了在解剖学上不同的肥大细胞群体中表现出显著差异的表型特征列表中。
