Gundersen H J, Jensen E B
J Microsc. 1985 May;138(Pt 2):127-42. doi: 10.1111/j.1365-2818.1985.tb02607.x.
A stereological estimator of the weighted mean volume of particles of arbitrary shape is described. This unbiased estimator is based on simple point-sampling of linear intercept lengths. The complete absence of shape assumptions effectively breaks the long-standing 'convexity-barrier': the only requirement here is that individual particles can be unambiguously identified by their profiles on random sections. Practical details of the simple estimation procedure and an example with very irregular particles are reported. Finally, an estimator of the variance of the weighted distribution of particle volume is discussed. This estimator is also valid for particles of arbitrary shape. For any mixture of ellipsoids (spheres, oblates, prolates and triaxial ellipsoids) the estimator is reduced to a simple function of measurements of diameters in the section plane.
本文描述了一种用于估计任意形状颗粒加权平均体积的体视学方法。该无偏估计器基于线性截距长度的简单点采样。完全不依赖形状假设有效地打破了长期存在的“凸性障碍”:这里唯一的要求是单个颗粒能够通过其在随机截面的轮廓被明确识别。报告了简单估计程序的实际细节以及一个关于非常不规则颗粒的示例。最后,讨论了颗粒体积加权分布方差的估计器。该估计器对任意形状的颗粒也有效。对于任何椭球体(球体、扁球体、长球体和三轴椭球体)的混合物,该估计器简化为截面平面中直径测量值的简单函数。