Gong Guanwen, Wang Pengfei, Ding Weiwei, Zhao Yunzhao, Li Jieshou
Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province, PR China.
Free Radic Res. 2009 May;43(5):470-7. doi: 10.1080/10715760902846157.
The purpose of this study was to study the impact of intra-abdominal hypertension (IAH) on the intestine.
One hundred and twenty Sprague-Daley rats were divided into four groups. In the ACS group, the intra-abdominal pressure (IAP) was increased to 20 mmHg. In the ACS/DE group, increased IAP was followed by decompression. In the control1 and control2 groups, the IAP remained unchanged. Malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH) and glutathione peroxidase (GSH-Px) enzymes of the intestine were measured. Additionally, ileal tissues were obtained for histopathological examinations and apoptosis detection. Liver, spleen and mesenteric lymph nodes were obtained for microbiological analysis.
In the presence of IAH, MDA and MPO were increased, while GSH and GSH-Px were decreased. Microbiological analysis suggested bacterial translocation across the gut. Morphological examinations indicated that the Chiu's score and apoptotic index in the ACS/DE group were the highest in the four groups.
Oxidative stress plays an important role in the intestinal damage and bacterial translocation in abdominal compartment syndrome. Additionally, the influence of oxygen free radicals occurs mainly during the period of reperfusion rather than during the IAH period.
本研究旨在探讨腹腔内高压（IAH）对肠道的影响。
将120只Sprague-Daley大鼠分为四组。在急性腹腔间隔室综合征（ACS）组中，腹腔内压力（IAP）升高至20 mmHg。在ACS/减压（DE）组中，IAP升高后进行减压。在对照1组和对照2组中，IAP保持不变。检测肠道的丙二醛（MDA）、髓过氧化物酶（MPO）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）酶。此外，获取回肠组织进行组织病理学检查和细胞凋亡检测。获取肝脏、脾脏和肠系膜淋巴结进行微生物学分析。
在存在IAH的情况下，MDA和MPO升高，而GSH和GSH-Px降低。微生物学分析提示细菌发生肠道移位。形态学检查表明，ACS/DE组的Chiu评分和凋亡指数在四组中最高。
氧化应激在腹腔间隔室综合征的肠道损伤和细菌移位中起重要作用。此外，氧自由基的影响主要发生在再灌注期而非IAH期。
