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Involvement of carbohydrates in the hardening of the zona pellucida of mouse oocytes.

作者信息

Dolci S, Bertolani M V, Canipari R, De Felici M

机构信息

Department of Public Health and Cellular Biology, II University of Rome, Italy.

出版信息

Cell Biol Int Rep. 1991 Jul;15(7):571-9. doi: 10.1016/0309-1651(91)90004-3.

Abstract

The effect of lectins with different saccharide specificity (ConA, LCA, DBA, WGA and PNA) on enzymatic digestion of the zona pellucida (ZP) of mouse oocytes was studied. All lectins tested, except PNA, induced ZP hardening with different degrees of efficiency. Moreover, extensive ZP digestion with mixed exoglycosidase prevented "spontaneous" ZP hardening. These observations suggest that changes of the carbohydrate moieties can be involved in the hardening of the zona pellucida of mouse oocytes.

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