卡纳毒素对细胞培养的影响。
Effect of canatoxin on cell cultures.
作者信息
Campos M M, Carlini C R, Guimarães J A, Marques-Silva V M, Rumjanek V M
机构信息
Basic Research Centre, Instituto Nacional de Cancer, Rio de Janeiro, Brazil.
出版信息
Cell Biol Int Rep. 1991 Jul;15(7):581-94. doi: 10.1016/0309-1651(91)90005-4.
Canatoxin, a toxic protein isolated from Canavalia ensiformis, was shown to inhibit DNA synthesis and to produce a cytolytic effect when added in vitro to various cells, in doses ranging from 50 to 500 nM. In this case no selectivity was found for a certain cell type, as both normal and transformed cells could be affected by the toxic protein. The cytostatic effect was irreversible upon removal of the toxic protein from the medium and could be fully attained after exposing the cells to canatoxin for only 30 min. The use of an in vitro cell culture system may allow for a better insight on the mode of action of canatoxin.
刀豆毒素，一种从刀豆中分离出的有毒蛋白质，当在体外以50至500纳摩尔的剂量添加到各种细胞中时，显示出抑制DNA合成并产生细胞溶解作用。在这种情况下，未发现对特定细胞类型有选择性，因为正常细胞和转化细胞都可能受到这种有毒蛋白质的影响。当从培养基中去除有毒蛋白质后，细胞抑制作用是不可逆的，并且在细胞仅暴露于刀豆毒素30分钟后就可以完全实现。使用体外细胞培养系统可能有助于更好地了解刀豆毒素的作用模式。
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