Xu Wen-ting, Bian Zhen-yu, Fan Qi-ming, Li Gang, Tang Ting-ting
Department of Orthopaedics, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, People's Republic of China.
Cancer Lett. 2009 Aug 18;281(1):32-41. doi: 10.1016/j.canlet.2009.02.022. Epub 2009 Apr 1.
In an effort to study the interaction between MSCs and osteosarcoma, we established an animal model of primary osteosarcoma in nude mice using Saos-2 cells. hMSCs, labeled with adv-GFP, were injected through the caudal vein. We observed that exogenous hMSCs targeted the osteosarcoma site and promoted its growth and pulmonary metastasis in vivo. To elucidate the underlying mechanisms, we employed transwell, neutralization antibody and MTT assays in vitro. hMSCs migrated toward the conditioned medium from Saos-2 cells, and SDF-1 was involved in this migration. Likewise, Saos-2 cells migrated toward the conditioned medium from hMSCs and CCL5 played an important role in this migration. Furthermore, proliferation of Saos-2 cells was enhanced by the conditioned medium from hMSCs and CCL5 was at least partly responsible for this enhancement.
为了研究间充质干细胞（MSCs）与骨肉瘤之间的相互作用，我们使用Saos-2细胞在裸鼠中建立了原发性骨肉瘤动物模型。通过尾静脉注射用腺病毒绿色荧光蛋白（adv-GFP）标记的人骨髓间充质干细胞（hMSCs）。我们观察到外源性hMSCs靶向骨肉瘤部位并促进其在体内的生长和肺转移。为了阐明潜在机制，我们在体外采用了Transwell实验、中和抗体实验和MTT实验。hMSCs向来自Saos-2细胞的条件培养基迁移，并且基质细胞衍生因子-1（SDF-1）参与了这种迁移。同样，Saos-2细胞向来自hMSCs的条件培养基迁移，并且趋化因子CCL5在这种迁移中起重要作用。此外，来自hMSCs的条件培养基增强了Saos-2细胞的增殖，并且CCL5至少部分地促成了这种增强作用。
