采用响应面法优化利用竹制废水生产酵母生物质的培养条件。
Optimization of culture conditions for production of yeast biomass using bamboo wastewater by response surface methodology.
作者信息
Li Xin, Ouyang Jia, Xu Yong, Chen Mu, Song Xiangyang, Yong Qiang, Yu Shiyuan
机构信息
Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, Jiangsu Province 210037, China.
出版信息
Bioresour Technol. 2009 Jul;100(14):3613-7. doi: 10.1016/j.biortech.2009.03.001. Epub 2009 Apr 1.
Response surface methodology (RSM) was applied to optimize culture conditions for the growth of Candida utilis with bamboo wastewater. A significant influence of initial pH, fermentation time and yeast extract on biomass of C. utilis was evaluated by Plackett-Burman design (PBD). These factors were further optimized using a central composite design (CCD) and RSM. A combination of initial pH 6.1, fermentation time 69 h and yeast extract 1.17 g/L was optimum for maximum biomass of C. utilis. A 1.7-fold enhancement of biomass of C. utilis was gained after optimization in shake-flask cultivation. The biomass of C. utilis reached 19.17 g/L in 3 L fermentor.
采用响应面法（RSM）优化产朊假丝酵母利用竹制废水生长的培养条件。通过Plackett-Burman设计（PBD）评估了初始pH值、发酵时间和酵母提取物对产朊假丝酵母生物量的显著影响。使用中心复合设计（CCD）和RSM对这些因素进行了进一步优化。初始pH值6.1、发酵时间69小时和酵母提取物1.17 g/L的组合对于产朊假丝酵母的最大生物量是最佳的。在摇瓶培养中优化后，产朊假丝酵母的生物量提高了1.7倍。在3 L发酵罐中，产朊假丝酵母的生物量达到了19.17 g/L。
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