Stradaioli G, Sylla L, Monaci M, Maiorino M
Department of Animal Science, University of Udine, via delle Scienze 208, 33100 Udine, Italy.
Theriogenology. 2009 Jul 1;72(1):91-8. doi: 10.1016/j.theriogenology.2009.02.006. Epub 2009 Apr 3.
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is a selenoperoxidase accounting for most of the selenium content in mammalian testis, which has been found to be linked to fertility in humans. In this study, we addressed the issue whether PHGPx content in spermatozoa could be a predictive index of fertilization capacity for sire selection in bulls. Measurement of PHGPx in spermatozoa of 92 yearling bulls of three different Italian breeds (Chianina, Romagnola, and Marchigiana) revealed the presence of two quite well separated populations. A PHGPx activity of 130 mU/mg separated the high-PHGPx group (H-PHGPx, n=73) from the low-PHGPx group (L-PHGPx, n=19). Forward motility was markedly higher in the H-PHGPx group, which also contained a lower percentage of detached heads, abnormal midpiece, and proximal droplets. On the other hand, differently from the human studies, no correlation was observed between PHGPx activity and number of spermatozoa in the ejaculate. Apart from sperm count, which typically differed among breeds, and number of detached heads in the L-PHGPx group, which correlated with higher sperm count, no other significant difference in seminal parameters among breeds was apparent. The assay for sperm PHGPx activity therefore emerges as a unique tool to evaluate semen quality for sire selection.
磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）是一种硒依赖过氧化物酶，占哺乳动物睾丸中硒含量的大部分，已发现其与人类生育能力有关。在本研究中，我们探讨了精子中PHGPx含量是否可作为公牛种用选择时受精能力的预测指标这一问题。对三个不同意大利品种（契安尼娜牛、罗马尼奥拉牛和马尔凯牛）的92头一岁公牛精子中的PHGPx进行测量，结果显示存在两个明显分离的群体。以130 mU/mg的PHGPx活性将高PHGPx组（H-PHGPx，n = 73）与低PHGPx组（L-PHGPx，n = 19）区分开来。H-PHGPx组的前向运动能力明显更高，该组中头部脱落、中段异常和近端液滴的百分比也更低。另一方面，与人类研究不同的是，未观察到PHGPx活性与射精中精子数量之间存在相关性。除了不同品种间通常存在差异的精子计数，以及L-PHGPx组中与较高精子计数相关的头部脱落数量外，不同品种间精液参数没有其他明显差异。因此，精子PHGPx活性检测成为评估种用选择精液质量的独特工具。
